MEN1 mutations and potentially MEN1-targeting miRNAs are responsible for menin deficiency in sporadic and MEN1 syndrome-associated primary hyperparathyroidism

Vince Kornél Grolmusz, Katalin Borka, Annamária Kövesdi, Kinga Németh, Katalin Balogh, Csaba Dékány, András Kiss, Anna Szentpéteri, Beatrix Sármán, A. Somogyi, Éva Csajbók, Zsuzsanna Valkusz, Miklós Tóth, P. Igaz, K. Rácz, A. Patócs

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8 Citations (Scopus)

Abstract

Inherited, germline mutations of menin-coding MEN1 gene cause multiple endocrine neoplasia type 1 (MEN1), while somatic MEN1 mutations are the sole main driver mutations in sporadic primary hyperparathyroidism (PHPT), suggesting that menin deficiency has a central role in the pathogenesis of PHPT. MiRNAs are small, noncoding RNAs posttranscriptionally regulating gene expression. Our aim was to investigate both the role of MEN1 mutations and potentially MEN1-targeting miRNAs as the underlying cause of menin deficiency in MEN1-associated and sporadic PHPT tissues. Fifty six PHPT tissues, including 16 MEN1-associated tissues, were evaluated. Diagnosis of MEN1 syndrome was based on identification of germline MEN1 mutations. In silico target prediction was used to identify miRNAs potentially targeting MEN1. Menin expression was determined by immunohistochemistry while expression of miRNAs was analyzed by quantitative real-time PCR. Sporadic PHPT tissues were subjected to somatic MEN1 mutation analysis as well. Lack of nuclear menin was identified in all MEN1-associated and in 28% of sporadic PHPT tissues. Somatic MEN1 mutations were found in 25% of sporadic PHPTs. The sensitivity and specificity of menin immunohistochemistry to detect a MEN1 mutation were 86 and 87%, respectively. Expression levels of hsa-miR-24 and hsa-miR-28 were higher in sporadic compared to MEN1-associated PHPT tissues; however, no difference in miRNA levels occurred between menin-positive and menin-negative PHPT tissues. Menin deficiency is the consequence of a MEN1 mutation in most menin-negative PHPT tissues. Elevated expression of hsa-miR-24 and hsa-miR-28 mark the first epigenetic changes observed between sporadic and MEN1-associated PHPT.

Original languageEnglish
Pages (from-to)401-411
Number of pages11
JournalVirchows Archiv
Volume471
Issue number3
DOIs
Publication statusPublished - Sep 1 2017

Keywords

  • Immunohistochemistry
  • MEN1
  • Menin
  • microRNA
  • Mutation
  • Primary hyperparathyroidism

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Grolmusz, V. K., Borka, K., Kövesdi, A., Németh, K., Balogh, K., Dékány, C., Kiss, A., Szentpéteri, A., Sármán, B., Somogyi, A., Csajbók, É., Valkusz, Z., Tóth, M., Igaz, P., Rácz, K., & Patócs, A. (2017). MEN1 mutations and potentially MEN1-targeting miRNAs are responsible for menin deficiency in sporadic and MEN1 syndrome-associated primary hyperparathyroidism. Virchows Archiv, 471(3), 401-411. https://doi.org/10.1007/s00428-017-2158-3