Membranes to reduce adherence of somatic embryos to the cell lift impeller of a bioreactor

Seppo Sorvari, Riitta Mäkeläinen, Katriina Ahanen, Otto Toldi

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Membranes less attractive to embryos were tested as a replacement for nylon screens to prevent adherence of somatic embryos, cell clusters and cells to different sites of the bioreactor, a feature considered undesirable in plant cell suspension cultures. The results showed that the loss of embryogenic cell-mass could be halved by using silicone or track membranes. For aeration purposes, these membranes are as satisfactory as nylon screens conventionally used in cell lift impellers.

Original languageEnglish
Title of host publicationLiquid Culture Systems for in vitro Plant Propagation
PublisherSpringer Netherlands
Pages117-125
Number of pages9
ISBN (Electronic)9781402032004
ISBN (Print)1402031998, 9781402031991
DOIs
Publication statusPublished - Jan 1 2005

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Keywords

  • Artificial seed
  • Dialysis membrane
  • Microseed
  • Nylon screen
  • Silicone membrane
  • Somatic embryogenesis
  • Somatic seed
  • Track membrane

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Sorvari, S., Mäkeläinen, R., Ahanen, K., & Toldi, O. (2005). Membranes to reduce adherence of somatic embryos to the cell lift impeller of a bioreactor. In Liquid Culture Systems for in vitro Plant Propagation (pp. 117-125). Springer Netherlands. https://doi.org/10.1007/1-4020-3200-5-7