Membrane transport in Caenorhabditis elegans: An essential role for VPS34 at the nuclear membrane

Lorenz Roggo, Vincent Bernard, A. Kovács, Ann M. Rose, Fabienne Savoy, Monique Zetka, Matthias P. Wymann, Fritz Müller

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Here we present a detailed genetic analysis of let.512/vps34 that encodes the Caenorhabditis elegans homologue of the yeast phosphatidylinositol 3-kinase Vps34p. LET-512/VPS34 has essential functions and is ubiquitously expressed in all tissues and developmental stages. It accumulates at a perinuclear region, and mutations in let-512/vps34 result in an expansion of the outer nuclear membrane as well as in a mislocalization and subsequent complete lack of expression of LRP-1, a C.elegans LDL receptor normally associated with the apical surface of hypodermal cells. Using a GFP::2xFYVE fusion protein we found that the phosphatidylinositol 3-phosphate (PtdIns 3-P) product of LET-512/VPS34 is associated with a multitude of intracellular membranes and vesicles located at the periphery, including endocytic vesicles. We propose that LET-512/VPS34 is required for membrane transport from the outer nuclear membrane towards the cell periphery. Thus, LET-512/VPS34 may regulate the secretory pathway in a much broader range of compartments than was previously suggested for the yeast orthologue.

Original languageEnglish
Pages (from-to)1673-1683
Number of pages11
JournalEMBO Journal
Volume21
Issue number7
DOIs
Publication statusPublished - Apr 2 2002

Fingerprint

Linear Energy Transfer
Nuclear Envelope
Caenorhabditis elegans
Membranes
Yeast
Yeasts
Phosphatidylinositol 3-Kinase
Transport Vesicles
Intracellular Membranes
LDL Receptors
Secretory Pathway
Fusion reactions
Tissue
Mutation
Proteins

Keywords

  • Caenorhabditis elegans
  • Membrane transport
  • PtdIns 3-kinase

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Membrane transport in Caenorhabditis elegans : An essential role for VPS34 at the nuclear membrane. / Roggo, Lorenz; Bernard, Vincent; Kovács, A.; Rose, Ann M.; Savoy, Fabienne; Zetka, Monique; Wymann, Matthias P.; Müller, Fritz.

In: EMBO Journal, Vol. 21, No. 7, 02.04.2002, p. 1673-1683.

Research output: Contribution to journalArticle

Roggo, L, Bernard, V, Kovács, A, Rose, AM, Savoy, F, Zetka, M, Wymann, MP & Müller, F 2002, 'Membrane transport in Caenorhabditis elegans: An essential role for VPS34 at the nuclear membrane', EMBO Journal, vol. 21, no. 7, pp. 1673-1683. https://doi.org/10.1093/emboj/21.7.1673
Roggo, Lorenz ; Bernard, Vincent ; Kovács, A. ; Rose, Ann M. ; Savoy, Fabienne ; Zetka, Monique ; Wymann, Matthias P. ; Müller, Fritz. / Membrane transport in Caenorhabditis elegans : An essential role for VPS34 at the nuclear membrane. In: EMBO Journal. 2002 ; Vol. 21, No. 7. pp. 1673-1683.
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