Membrane permeability changes induce hyperpolarization in transformed lymphoid cells under high-density culture conditions

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Abstract

Background: Membrane potential changes in cells from the human lymphoid B cell line, JY, evoked by increasing cell density in culture were investigated, as data published on other cell types are controversial. An attempt was also made to clear the underlying mechanism. Methods: Nonadherent JY cells were isolated from high-density plateau-phase cultures (type A cells), medium-density log-phase cultures (type B cells), and low-density lag-phase cultures (type C cells). They were analyzed for transmembrane potential, intracellular free concentration of potassium and sodium, membrane permeability for monovalent cations, cell cycle distribution by measuring DNA content, and glucose uptake. Results: C type cells proved to be relatively depolarized (-41 ± 3 mV) and cells obtained from the highest density cultures hyperpolarized (-60 ± 3 mV). Intracellular concentrations ([K](i) = 92-97 mM and [Na](i) = 34-35 mM) were almost identical for each type of cell. The sodium/potassium permeability constant ratio in the A and C type of cells was 0.047 and 0.094, respectively. High-density culture conditions resulted in a pronounced G1-phase arrest. Conclusions: Differences in the membrane potential values induced by high-density culture conditions were maintained by changes in the membrane permeability for the monovalent cations. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)186-192
Number of pages7
JournalCytometry
Volume41
Issue number3
DOIs
Publication statusPublished - Nov 1 2000

Fingerprint

Permeability
Lymphocytes
Membranes
Membrane Potentials
Monovalent Cations
Cell Count
Potassium
B-Lymphocytes
Sodium
G1 Phase
Cell Cycle
Cell Culture Techniques
Glucose
Cell Line
DNA

Keywords

  • Cell density
  • G phase arrest
  • Glucose metabolism
  • Membrane permeability
  • Membrane potential

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

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title = "Membrane permeability changes induce hyperpolarization in transformed lymphoid cells under high-density culture conditions",
abstract = "Background: Membrane potential changes in cells from the human lymphoid B cell line, JY, evoked by increasing cell density in culture were investigated, as data published on other cell types are controversial. An attempt was also made to clear the underlying mechanism. Methods: Nonadherent JY cells were isolated from high-density plateau-phase cultures (type A cells), medium-density log-phase cultures (type B cells), and low-density lag-phase cultures (type C cells). They were analyzed for transmembrane potential, intracellular free concentration of potassium and sodium, membrane permeability for monovalent cations, cell cycle distribution by measuring DNA content, and glucose uptake. Results: C type cells proved to be relatively depolarized (-41 ± 3 mV) and cells obtained from the highest density cultures hyperpolarized (-60 ± 3 mV). Intracellular concentrations ([K](i) = 92-97 mM and [Na](i) = 34-35 mM) were almost identical for each type of cell. The sodium/potassium permeability constant ratio in the A and C type of cells was 0.047 and 0.094, respectively. High-density culture conditions resulted in a pronounced G1-phase arrest. Conclusions: Differences in the membrane potential values induced by high-density culture conditions were maintained by changes in the membrane permeability for the monovalent cations. (C) 2000 Wiley-Liss, Inc.",
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author = "T. M{\'a}ri{\'a}n and L. Balkay and Z. Krasznai and L. Tr{\'o}n",
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AU - Krasznai, Z.

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N2 - Background: Membrane potential changes in cells from the human lymphoid B cell line, JY, evoked by increasing cell density in culture were investigated, as data published on other cell types are controversial. An attempt was also made to clear the underlying mechanism. Methods: Nonadherent JY cells were isolated from high-density plateau-phase cultures (type A cells), medium-density log-phase cultures (type B cells), and low-density lag-phase cultures (type C cells). They were analyzed for transmembrane potential, intracellular free concentration of potassium and sodium, membrane permeability for monovalent cations, cell cycle distribution by measuring DNA content, and glucose uptake. Results: C type cells proved to be relatively depolarized (-41 ± 3 mV) and cells obtained from the highest density cultures hyperpolarized (-60 ± 3 mV). Intracellular concentrations ([K](i) = 92-97 mM and [Na](i) = 34-35 mM) were almost identical for each type of cell. The sodium/potassium permeability constant ratio in the A and C type of cells was 0.047 and 0.094, respectively. High-density culture conditions resulted in a pronounced G1-phase arrest. Conclusions: Differences in the membrane potential values induced by high-density culture conditions were maintained by changes in the membrane permeability for the monovalent cations. (C) 2000 Wiley-Liss, Inc.

AB - Background: Membrane potential changes in cells from the human lymphoid B cell line, JY, evoked by increasing cell density in culture were investigated, as data published on other cell types are controversial. An attempt was also made to clear the underlying mechanism. Methods: Nonadherent JY cells were isolated from high-density plateau-phase cultures (type A cells), medium-density log-phase cultures (type B cells), and low-density lag-phase cultures (type C cells). They were analyzed for transmembrane potential, intracellular free concentration of potassium and sodium, membrane permeability for monovalent cations, cell cycle distribution by measuring DNA content, and glucose uptake. Results: C type cells proved to be relatively depolarized (-41 ± 3 mV) and cells obtained from the highest density cultures hyperpolarized (-60 ± 3 mV). Intracellular concentrations ([K](i) = 92-97 mM and [Na](i) = 34-35 mM) were almost identical for each type of cell. The sodium/potassium permeability constant ratio in the A and C type of cells was 0.047 and 0.094, respectively. High-density culture conditions resulted in a pronounced G1-phase arrest. Conclusions: Differences in the membrane potential values induced by high-density culture conditions were maintained by changes in the membrane permeability for the monovalent cations. (C) 2000 Wiley-Liss, Inc.

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