Membrane lipid perturbation modifies the set point of the temperature of heat shock response in yeast

Luisella Carratù, Silvia Franceschelli, Celia L. Pardini, George S. Kobayashi, Ibolya Horvath, Laszlo Vigh, Bruno Maresca

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Abstract

Addition of a saturated fatty acid (SFA) induced a strong increase in heat shock (HS) mRNA transcription when cells were heat-shocked at 37°C, whereas treatment with an unsaturated fatty acid (UFA) reduced or eliminated the level of HS gene transcription at 37°C. Transcription of the Δ9-desaturase gene (Ole1) of Histoplasma capsulatum, whose gene product is responsible for the synthesis of UFA, is up-regulated in a temperature-sensitive strain. We show that when the L8-14C mutant of Saccharomyces cerevisiae, which has a disrupted Ole1 gene, is complemented with its own Ole1 coding region under control of its own promoter or Ole1 promoters of H. capsulatum, the level of HS gene transcription depends on the activity of the promoters. Fluorescence anisotropy of mitochondrial membranes of complemented strains corresponded to the different activity of the Ole1 promoter used. We propose that the SFA/UFA ratio and perturbation of membrane lipoprotein complexes are involved in the perception of rapid temperature changes and under HS conditions disturbance of the preexisting membrane physical state causes transduction of a signal that induces transcription of HS genes.

Original languageEnglish
Pages (from-to)3870-3875
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number9
DOIs
Publication statusPublished - Apr 30 1996

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Keywords

  • membrane physical state
  • Δ-desaturase

ASJC Scopus subject areas

  • General

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