Membrane glucocorticoid receptor expression on peripheral blood mononuclear cells in patients with ankylosing spondylitis

Anita B. Tryc, Cornelia M. Spies, Udo Schneider, Desiree Kunkel, T. Berki, Joachim Sieper, Gerd Rüdiger Burmester, Andreas Radbruch, Alexander Scheffold, Frank Buttgereit

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective. To investigate the expression of membrane glucocorticoid receptors (mGCR) on peripheral blood mononuclear cells (PBMC) in patients with ankylosing spondylitis (AS). Methods. We used high sensitivity immunofluorescence with magnetofluorescent liposomes for the detection of mGCR on PBMC from patients with AS (n = 26) and healthy controls (n = 11). Results. The frequency of mGCR+ monocytes and B lymphocytes was significantly higher in patients with AS than in controls (monocytes 12.5 ± 9.9% vs 4.8 ± 1.4%. B lymphocytes 8.7 ± 6.3% vs 4.4 ± 3.6%). We did not find mGCR on T lymphocytes. The frequency of mGCR+ cells did not correlate with variables of AS disease activity [C-reactive protein, erythrocyte sedimentation rate. Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), BASDAI 6, or numerical rating scales]. Conclusion. mGCR are upregulated in monocytes and B lymphocytes of patients with AS. This upregulation does not correlate with the humoral or overall disease activity. mGCR are not present on T lymphocytes. Our findings may be related to the limited benefit of low-dose and the efficacy of high-dose (intravenous pulse or intraarticular) glucocorticoid treatment in AS. Drugs binding selectively to mGCR may be a new therapeutic option for AS.

Original languageEnglish
Pages (from-to)2249-2253
Number of pages5
JournalJournal of Rheumatology
Volume33
Issue number11
Publication statusPublished - Nov 2006

Fingerprint

Ankylosing Spondylitis
Glucocorticoid Receptors
Blood Cells
Membranes
Monocytes
B-Lymphocytes
Baths
T-Lymphocytes
Blood Sedimentation
Liposomes
C-Reactive Protein
Glucocorticoids
Fluorescent Antibody Technique
Up-Regulation
Therapeutics

Keywords

  • Ankylosing spondylitis
  • Glucocorticoid
  • Membrane receptors
  • Nongenomic
  • Peripheral blood mononuclear cells

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Tryc, A. B., Spies, C. M., Schneider, U., Kunkel, D., Berki, T., Sieper, J., ... Buttgereit, F. (2006). Membrane glucocorticoid receptor expression on peripheral blood mononuclear cells in patients with ankylosing spondylitis. Journal of Rheumatology, 33(11), 2249-2253.

Membrane glucocorticoid receptor expression on peripheral blood mononuclear cells in patients with ankylosing spondylitis. / Tryc, Anita B.; Spies, Cornelia M.; Schneider, Udo; Kunkel, Desiree; Berki, T.; Sieper, Joachim; Burmester, Gerd Rüdiger; Radbruch, Andreas; Scheffold, Alexander; Buttgereit, Frank.

In: Journal of Rheumatology, Vol. 33, No. 11, 11.2006, p. 2249-2253.

Research output: Contribution to journalArticle

Tryc, AB, Spies, CM, Schneider, U, Kunkel, D, Berki, T, Sieper, J, Burmester, GR, Radbruch, A, Scheffold, A & Buttgereit, F 2006, 'Membrane glucocorticoid receptor expression on peripheral blood mononuclear cells in patients with ankylosing spondylitis', Journal of Rheumatology, vol. 33, no. 11, pp. 2249-2253.
Tryc, Anita B. ; Spies, Cornelia M. ; Schneider, Udo ; Kunkel, Desiree ; Berki, T. ; Sieper, Joachim ; Burmester, Gerd Rüdiger ; Radbruch, Andreas ; Scheffold, Alexander ; Buttgereit, Frank. / Membrane glucocorticoid receptor expression on peripheral blood mononuclear cells in patients with ankylosing spondylitis. In: Journal of Rheumatology. 2006 ; Vol. 33, No. 11. pp. 2249-2253.
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AU - Sieper, Joachim

AU - Burmester, Gerd Rüdiger

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