Membrane fluidization triggers membrane remodeling which affects the thermotolerance in Escherichia coli

Natalia Shigapova, Z. Török, G. Balogh, Pierre Goloubinoff, L. Vígh, I. Horváth

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Treatment of Escherichia coli with non-lethal doses of heat or benzyl alcohol (BA) causes transient membrane fluidization and permeabilization, and induces the rapid transcription of heat-shock genes in a σ32- dependent manner. This early response is followed by a rapid adaptation (priming) of the cells to otherwise lethal elevated temperature, in strong correlation with an observed remodeling of the composition and alkyl chain unsaturation of membrane lipids. The acquisition of cellular thermotolerance in BA-primed cells is unrelated to protein denaturation and is not accompanied by the formation of major heat-shock proteins, such as GroEL and DnaK. This suggests that the rapid remodeling of membrane composition is sufficient for the short-term bacterial thermotolerance.

Original languageEnglish
Pages (from-to)1216-1223
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume328
Issue number4
DOIs
Publication statusPublished - Mar 25 2005

Fingerprint

Benzyl Alcohol
Fluidization
Escherichia coli
Hot Temperature
Membranes
Protein Denaturation
Denaturation
Transcription
Membrane Lipids
Heat-Shock Proteins
Chemical analysis
Shock
Genes
Temperature
Proteins
Thermotolerance

Keywords

  • Acquired thermotolerance
  • Heat stress
  • Lipid saturation
  • Membrane fluidity
  • Molecular chaperones
  • Thermosensor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Membrane fluidization triggers membrane remodeling which affects the thermotolerance in Escherichia coli. / Shigapova, Natalia; Török, Z.; Balogh, G.; Goloubinoff, Pierre; Vígh, L.; Horváth, I.

In: Biochemical and Biophysical Research Communications, Vol. 328, No. 4, 25.03.2005, p. 1216-1223.

Research output: Contribution to journalArticle

@article{39b3217712d74bc497cdb9040f9cb529,
title = "Membrane fluidization triggers membrane remodeling which affects the thermotolerance in Escherichia coli",
abstract = "Treatment of Escherichia coli with non-lethal doses of heat or benzyl alcohol (BA) causes transient membrane fluidization and permeabilization, and induces the rapid transcription of heat-shock genes in a σ32- dependent manner. This early response is followed by a rapid adaptation (priming) of the cells to otherwise lethal elevated temperature, in strong correlation with an observed remodeling of the composition and alkyl chain unsaturation of membrane lipids. The acquisition of cellular thermotolerance in BA-primed cells is unrelated to protein denaturation and is not accompanied by the formation of major heat-shock proteins, such as GroEL and DnaK. This suggests that the rapid remodeling of membrane composition is sufficient for the short-term bacterial thermotolerance.",
keywords = "Acquired thermotolerance, Heat stress, Lipid saturation, Membrane fluidity, Molecular chaperones, Thermosensor",
author = "Natalia Shigapova and Z. T{\"o}r{\"o}k and G. Balogh and Pierre Goloubinoff and L. V{\'i}gh and I. Horv{\'a}th",
year = "2005",
month = "3",
day = "25",
doi = "10.1016/j.bbrc.2005.01.081",
language = "English",
volume = "328",
pages = "1216--1223",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Membrane fluidization triggers membrane remodeling which affects the thermotolerance in Escherichia coli

AU - Shigapova, Natalia

AU - Török, Z.

AU - Balogh, G.

AU - Goloubinoff, Pierre

AU - Vígh, L.

AU - Horváth, I.

PY - 2005/3/25

Y1 - 2005/3/25

N2 - Treatment of Escherichia coli with non-lethal doses of heat or benzyl alcohol (BA) causes transient membrane fluidization and permeabilization, and induces the rapid transcription of heat-shock genes in a σ32- dependent manner. This early response is followed by a rapid adaptation (priming) of the cells to otherwise lethal elevated temperature, in strong correlation with an observed remodeling of the composition and alkyl chain unsaturation of membrane lipids. The acquisition of cellular thermotolerance in BA-primed cells is unrelated to protein denaturation and is not accompanied by the formation of major heat-shock proteins, such as GroEL and DnaK. This suggests that the rapid remodeling of membrane composition is sufficient for the short-term bacterial thermotolerance.

AB - Treatment of Escherichia coli with non-lethal doses of heat or benzyl alcohol (BA) causes transient membrane fluidization and permeabilization, and induces the rapid transcription of heat-shock genes in a σ32- dependent manner. This early response is followed by a rapid adaptation (priming) of the cells to otherwise lethal elevated temperature, in strong correlation with an observed remodeling of the composition and alkyl chain unsaturation of membrane lipids. The acquisition of cellular thermotolerance in BA-primed cells is unrelated to protein denaturation and is not accompanied by the formation of major heat-shock proteins, such as GroEL and DnaK. This suggests that the rapid remodeling of membrane composition is sufficient for the short-term bacterial thermotolerance.

KW - Acquired thermotolerance

KW - Heat stress

KW - Lipid saturation

KW - Membrane fluidity

KW - Molecular chaperones

KW - Thermosensor

UR - http://www.scopus.com/inward/record.url?scp=13744256921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13744256921&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2005.01.081

DO - 10.1016/j.bbrc.2005.01.081

M3 - Article

C2 - 15708006

AN - SCOPUS:13744256921

VL - 328

SP - 1216

EP - 1223

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -