Membrane fluidity of trophoblast cells and susceptibility to natural cytotoxicity

J. Szekeres-Barthó, A. Nemeth, P. Varga, V. Csernus, T. Kőszegi, M. Paal

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This study examines the relationship between membrane lipid microviscosity and susceptibility of villous trophoblast to lysis by natural cytotoxic cells. Trophoblast-enriched cell suspensions prepared from term human placentae were treated with cholesteryl hemisuccinate (CHS) - a modulator of membrane lipid microviscosity. CHS-treated cells were more susceptible targets for natural lymphocyte cytotoxicity than were untreated controls. In binding experiments, increased binding of lymphocytes to CHS-treated target cells was found. Preincubation with progesterone prevented membrane rigidification by CHS. Progesterone, cortisol, and estriol restored the impaired resistance of CHS-treated trophoblast cells to lysis. We determined microviscosity and progesterone concentration in villous surface membranes, prepared from placentae from idiopathic spontaneous abortions and normal first-trimester pregnancies. An inverse relationship was found between progesterone content and microviscosity of the membranes. Microviscosity of the membranes from abortion placentae was significantly higher (P <.01) and progesterone concentration was significantly lower (P <.001) than those in the membranes of normal first trimester placentae.

Original languageEnglish
Pages (from-to)92-98
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume19
Issue number3
Publication statusPublished - 1989

Fingerprint

Membrane Fluidity
Trophoblasts
Progesterone
Placenta
Membranes
First Pregnancy Trimester
Membrane Lipids
Lymphocytes
Estriol
Spontaneous Abortion
Hydrocortisone
Suspensions
cholesteryl succinate

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynaecology

Cite this

Membrane fluidity of trophoblast cells and susceptibility to natural cytotoxicity. / Szekeres-Barthó, J.; Nemeth, A.; Varga, P.; Csernus, V.; Kőszegi, T.; Paal, M.

In: American Journal of Reproductive Immunology, Vol. 19, No. 3, 1989, p. 92-98.

Research output: Contribution to journalArticle

@article{06b4a9bf72f4487a821004a8b3d1756e,
title = "Membrane fluidity of trophoblast cells and susceptibility to natural cytotoxicity",
abstract = "This study examines the relationship between membrane lipid microviscosity and susceptibility of villous trophoblast to lysis by natural cytotoxic cells. Trophoblast-enriched cell suspensions prepared from term human placentae were treated with cholesteryl hemisuccinate (CHS) - a modulator of membrane lipid microviscosity. CHS-treated cells were more susceptible targets for natural lymphocyte cytotoxicity than were untreated controls. In binding experiments, increased binding of lymphocytes to CHS-treated target cells was found. Preincubation with progesterone prevented membrane rigidification by CHS. Progesterone, cortisol, and estriol restored the impaired resistance of CHS-treated trophoblast cells to lysis. We determined microviscosity and progesterone concentration in villous surface membranes, prepared from placentae from idiopathic spontaneous abortions and normal first-trimester pregnancies. An inverse relationship was found between progesterone content and microviscosity of the membranes. Microviscosity of the membranes from abortion placentae was significantly higher (P <.01) and progesterone concentration was significantly lower (P <.001) than those in the membranes of normal first trimester placentae.",
author = "J. Szekeres-Barth{\'o} and A. Nemeth and P. Varga and V. Csernus and T. Kőszegi and M. Paal",
year = "1989",
language = "English",
volume = "19",
pages = "92--98",
journal = "American Journal of Reproductive Immunology",
issn = "8755-8920",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Membrane fluidity of trophoblast cells and susceptibility to natural cytotoxicity

AU - Szekeres-Barthó, J.

AU - Nemeth, A.

AU - Varga, P.

AU - Csernus, V.

AU - Kőszegi, T.

AU - Paal, M.

PY - 1989

Y1 - 1989

N2 - This study examines the relationship between membrane lipid microviscosity and susceptibility of villous trophoblast to lysis by natural cytotoxic cells. Trophoblast-enriched cell suspensions prepared from term human placentae were treated with cholesteryl hemisuccinate (CHS) - a modulator of membrane lipid microviscosity. CHS-treated cells were more susceptible targets for natural lymphocyte cytotoxicity than were untreated controls. In binding experiments, increased binding of lymphocytes to CHS-treated target cells was found. Preincubation with progesterone prevented membrane rigidification by CHS. Progesterone, cortisol, and estriol restored the impaired resistance of CHS-treated trophoblast cells to lysis. We determined microviscosity and progesterone concentration in villous surface membranes, prepared from placentae from idiopathic spontaneous abortions and normal first-trimester pregnancies. An inverse relationship was found between progesterone content and microviscosity of the membranes. Microviscosity of the membranes from abortion placentae was significantly higher (P <.01) and progesterone concentration was significantly lower (P <.001) than those in the membranes of normal first trimester placentae.

AB - This study examines the relationship between membrane lipid microviscosity and susceptibility of villous trophoblast to lysis by natural cytotoxic cells. Trophoblast-enriched cell suspensions prepared from term human placentae were treated with cholesteryl hemisuccinate (CHS) - a modulator of membrane lipid microviscosity. CHS-treated cells were more susceptible targets for natural lymphocyte cytotoxicity than were untreated controls. In binding experiments, increased binding of lymphocytes to CHS-treated target cells was found. Preincubation with progesterone prevented membrane rigidification by CHS. Progesterone, cortisol, and estriol restored the impaired resistance of CHS-treated trophoblast cells to lysis. We determined microviscosity and progesterone concentration in villous surface membranes, prepared from placentae from idiopathic spontaneous abortions and normal first-trimester pregnancies. An inverse relationship was found between progesterone content and microviscosity of the membranes. Microviscosity of the membranes from abortion placentae was significantly higher (P <.01) and progesterone concentration was significantly lower (P <.001) than those in the membranes of normal first trimester placentae.

UR - http://www.scopus.com/inward/record.url?scp=0024406963&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024406963&partnerID=8YFLogxK

M3 - Article

VL - 19

SP - 92

EP - 98

JO - American Journal of Reproductive Immunology

JF - American Journal of Reproductive Immunology

SN - 8755-8920

IS - 3

ER -