Membrane depolarization selectively inhibits receptor-operated calcium channels in human T (Jurkat) lymphoblasts

Research output: Contribution to journalArticle

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Abstract

Jurkat lymphoblasts were stimulated by a monoclonal antibody against the CD3 membrane antigen and the evoked calcium signal was followed by the intracellular fluorescent calcium indicator indo-1. The technique applied allowed us to separately investigate the stimulus-induced intracellular calcium release and the calcium-influx pathways, respectively. In the same cells membrane potential was estimated by the fluorescent dye diS-C3-(5). The resting membrane potential of Jurkat lymphoblasts under normal conditions was between -55 and -60 mV. Membrane depolarization, obtained by increasing external K+ concentration, removing external Cl-, or by increasing the Na+/K+ leak permeability with gramicidin or PCMBS, did not induce calcium influx in the resting cells and did not influence the CD3 receptor-mediated internal calcium release, while strongly inhibited the receptor-mediated calcium influx pathway. Half-maximum inhibition of this calcium influx was observed at membrane potential values of about -35 to -40 mV and this inhibition did not depend on the external calcium concentration varied between 5 and 2500 μM. Membrane hyperpolarization by valinomycin did not affect either component of the calcium signal. The observed selective inhibition of the receptor-operated calcium influx pathway by membrane depolarization is probably an important modulator of calcium-dependent cell stimulation.

Original languageEnglish
Pages (from-to)130-140
Number of pages11
JournalBBA - Biomembranes
Volume1027
Issue number2
DOIs
Publication statusPublished - Aug 24 1990

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Depolarization
Calcium Channels
Calcium
Membranes
Membrane Potentials
Calcium-Sensing Receptors
4-Chloromercuribenzenesulfonate
CD3 Antigens
Valinomycin
Gramicidin
Fluorescent Dyes
Cell membranes
Permeability
Modulators
Monoclonal Antibodies
Cell Membrane
Antigens

Keywords

  • Calcium ion influx
  • Calcium release
  • Calcium signal
  • Membrane potential
  • Receptor-operated calcium channel
  • T (Jurkat) lymphoblast

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Membrane depolarization selectively inhibits receptor-operated calcium channels in human T (Jurkat) lymphoblasts. / Sarkadi, Balázs; Tordai, Attila; Gárdos, George.

In: BBA - Biomembranes, Vol. 1027, No. 2, 24.08.1990, p. 130-140.

Research output: Contribution to journalArticle

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