Membrane depolarization inhibits thrombin-induced calcium influx and aggregation in human platelets

Tünde Kovács, A. Tordai, Ilma Szász, B. Sarkadi, G. Gárdos

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The relationship between thrombin-evoked changes in intracellular calcium concentration ([Ca2+]i) and aggregation was examined in Indo-1-loaded human platelets. The stimulus-induced intracellular calcium release and external calcium influx, as well as platelet aggregation, were studied in the same cell preparation. A close correlation between the sustained high [Ca2+]i level, depending on calcium entry, and the aggregation response was found. Gramicidin, at a concentration high enough to induce membrane depolarization, strongly inhibited the calcium influx and aggregation, but did not influence the thrombin-induced intracellular calcium release. We conclude that calcium influx through depolarization-inhibited calcium channels is a prerequisite of thrombin-induced platelet aggregation.

Original languageEnglish
Pages (from-to)171-174
Number of pages4
JournalFEBS letters
Volume266
Issue number1-2
DOIs
Publication statusPublished - Jun 18 1990

Keywords

  • Aggregation
  • Calcium flux
  • Human platelet
  • Membrane potential
  • Receptor-operated calcium channel
  • Thrombin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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