Membrane binding properties of IRSp53-missing in metastasis domain (IMD) protein

Kinga Futó, Emoke Bódis, Laura M. Machesky, Miklós Nyitrai, Balázs Visegrády

Research output: Contribution to journalArticle

4 Citations (Scopus)


The 53-kDa insulin receptor substrate protein (IRSp53) organizes the actin cytoskeleton in response to stimulation of small GTPases, promoting the formation of cell protrusions such as filopodia and lamellipodia. IMD is the N-terminal 250 amino acid domain (IRSp53/MIM Homology Domain) of IRSp53 (also called I-BAR), which can bind to negatively charged lipid molecules. Overexpression of IMD induces filopodia formation in cells and purified IMD assembles finger-like protrusions in reconstituted lipid membranes. IMD was shown by several groups to bundle actin filaments, but other groups showed that it also binds to membranes. IMD binds to negatively charged lipid molecules with preference to clusters of PI(4,5)P2. Here, we performed a range of different in vitro fluorescence experiments to determine the binding properties of the IMD to phospholipids. We used different constructs of large unilamellar vesicles (LUVETs), containing neutral or negatively charged phospholipids. We found that IMD has a stronger binding interaction with negatively charged PI(4,5)P2 or PS lipids than PS/PC or neutral PC lipids. The equilibrium dissociation constant for the IMD-lipid interaction falls into the 78-170 μM range for all the lipids tested. The solvent accessibility of the fluorescence labels on the IMD during its binding to lipids is also reduced as the lipids become more negatively charged. Actin affects the IMD-lipid interaction, depending on its polymerization state. Monomeric actin partially disrupts the binding, while filamentous actin can further stabilize the IMD-lipid interaction.

Original languageEnglish
Pages (from-to)1651-1655
Number of pages5
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Issue number11
Publication statusPublished - Sep 26 2013



  • Actin
  • Fluorescence spectroscopy
  • I-BAR
  • IMD
  • IRSp53

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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