Melanoma cell-derived exosomes alter macrophage and dendritic cell functions in vitro

Annamaria Marton, Csaba Vizler, Erzsebet Kusz, Viktoria Temesfoi, Zsuzsa Szathmary, Krisztina Nagy, Zsolt Szegletes, Gyorgy Varo, Laszlo Siklos, Robert L. Katona, Vilmos Tubak, O. M.Zack Howard, Erno Duda, Janos Minarovits, Katalin Nagy, Krisztina Buzas

Research output: Contribution to journalArticle

60 Citations (Scopus)


To clarify controversies in the literature of the field, we have purified and characterized B16F1 melanoma cell derived exosomes (mcd-exosomes) then we attempted to dissect their immunological activities. We tested how mcd-exosomes influence CD4+ T cell proliferation induced by bone marrow derived dendritic cells; we quantified NF-κB activation in mature macrophages stimulated with mcd-exosomes, and we compared the cytokine profile of LPS-stimulated, IL-4 induced, and mcd-exosome treated macrophages. We observed that mcd-exosomes helped the maturation of dendritic cells, enhancing T cell proliferation induced by the treated dendritic cells. The exosomes also activated macrophages, as measured by NF-κB activation. The cytokine and chemokine profile of macrophages treated with tumor cell derived exosomes showed marked differences from those induced by either LPS or IL-4, and it suggested that exosomes may play a role in the tumor progression and metastasis formation through supporting tumor immune escape mechanisms.

Original languageEnglish
Pages (from-to)34-38
Number of pages5
JournalImmunology letters
Issue number1
Publication statusPublished - Nov 1 2012


  • Cytokine profile
  • Exosomes
  • Melanoma
  • Tumor immunity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Melanoma cell-derived exosomes alter macrophage and dendritic cell functions in vitro'. Together they form a unique fingerprint.

  • Cite this