Meiotic progression, mitochondrial features and fertilisation characteristics of porcine oocytes with different G6PDH activities

I. Egerszegi, Hannelore Alm, J. Rátky, Bassiouni Heleil, Klaus Peter Brüssow, Helmut Torner

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The aim of the present study was to investigate the developmental competence, mitochondrial characteristics and chromatin status of immature follicular porcine oocytes selected for their glucose-6-phosphate dehydrogenase (G6PDH) activity by brilliant cresyl blue (BCB) staining. In Experiment 1, the oocyte parameters were determined in parallel right after BCB staining (T 0), after 22 h of in vitro maturation (IVM) (T22) and after 44 h of IVM (T44) (n=496). BCB-stained oocytes (BCB+) at T 0 were characterised by fibrillated chromatin filaments in their germinal vesicles (GV) and diakinesis stages whereas unstained (BCB-) oocytes at T0 contained in their GV mainly condensed stages of chromatin (P

Original languageEnglish
Pages (from-to)830-838
Number of pages9
JournalReproduction, Fertility and Development
Volume22
Issue number5
DOIs
Publication statusPublished - 2010

Fingerprint

Glucosephosphate Dehydrogenase
glucose-6-phosphate 1-dehydrogenase
Fertilization
Oocytes
oocytes
Swine
chromatin
germinal vesicle
swine
Chromatin
Staining and Labeling
Meiotic Prophase I
prophase
immatures
Mental Competency
Brilliant Cresyl Blue
staining
In Vitro Techniques

Keywords

  • Mitochondria
  • Pig

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology
  • Reproductive Medicine
  • Animal Science and Zoology

Cite this

Meiotic progression, mitochondrial features and fertilisation characteristics of porcine oocytes with different G6PDH activities. / Egerszegi, I.; Alm, Hannelore; Rátky, J.; Heleil, Bassiouni; Brüssow, Klaus Peter; Torner, Helmut.

In: Reproduction, Fertility and Development, Vol. 22, No. 5, 2010, p. 830-838.

Research output: Contribution to journalArticle

@article{39dad258791a42e8a73ca370677b6fb1,
title = "Meiotic progression, mitochondrial features and fertilisation characteristics of porcine oocytes with different G6PDH activities",
abstract = "The aim of the present study was to investigate the developmental competence, mitochondrial characteristics and chromatin status of immature follicular porcine oocytes selected for their glucose-6-phosphate dehydrogenase (G6PDH) activity by brilliant cresyl blue (BCB) staining. In Experiment 1, the oocyte parameters were determined in parallel right after BCB staining (T 0), after 22 h of in vitro maturation (IVM) (T22) and after 44 h of IVM (T44) (n=496). BCB-stained oocytes (BCB+) at T 0 were characterised by fibrillated chromatin filaments in their germinal vesicles (GV) and diakinesis stages whereas unstained (BCB-) oocytes at T0 contained in their GV mainly condensed stages of chromatin (P",
keywords = "Mitochondria, Pig",
author = "I. Egerszegi and Hannelore Alm and J. R{\'a}tky and Bassiouni Heleil and Br{\"u}ssow, {Klaus Peter} and Helmut Torner",
year = "2010",
doi = "10.1071/RD09140",
language = "English",
volume = "22",
pages = "830--838",
journal = "Reproduction, Fertility and Development",
issn = "1031-3613",
publisher = "CSIRO",
number = "5",

}

TY - JOUR

T1 - Meiotic progression, mitochondrial features and fertilisation characteristics of porcine oocytes with different G6PDH activities

AU - Egerszegi, I.

AU - Alm, Hannelore

AU - Rátky, J.

AU - Heleil, Bassiouni

AU - Brüssow, Klaus Peter

AU - Torner, Helmut

PY - 2010

Y1 - 2010

N2 - The aim of the present study was to investigate the developmental competence, mitochondrial characteristics and chromatin status of immature follicular porcine oocytes selected for their glucose-6-phosphate dehydrogenase (G6PDH) activity by brilliant cresyl blue (BCB) staining. In Experiment 1, the oocyte parameters were determined in parallel right after BCB staining (T 0), after 22 h of in vitro maturation (IVM) (T22) and after 44 h of IVM (T44) (n=496). BCB-stained oocytes (BCB+) at T 0 were characterised by fibrillated chromatin filaments in their germinal vesicles (GV) and diakinesis stages whereas unstained (BCB-) oocytes at T0 contained in their GV mainly condensed stages of chromatin (P

AB - The aim of the present study was to investigate the developmental competence, mitochondrial characteristics and chromatin status of immature follicular porcine oocytes selected for their glucose-6-phosphate dehydrogenase (G6PDH) activity by brilliant cresyl blue (BCB) staining. In Experiment 1, the oocyte parameters were determined in parallel right after BCB staining (T 0), after 22 h of in vitro maturation (IVM) (T22) and after 44 h of IVM (T44) (n=496). BCB-stained oocytes (BCB+) at T 0 were characterised by fibrillated chromatin filaments in their germinal vesicles (GV) and diakinesis stages whereas unstained (BCB-) oocytes at T0 contained in their GV mainly condensed stages of chromatin (P

KW - Mitochondria

KW - Pig

UR - http://www.scopus.com/inward/record.url?scp=77951844544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951844544&partnerID=8YFLogxK

U2 - 10.1071/RD09140

DO - 10.1071/RD09140

M3 - Article

C2 - 20450835

AN - SCOPUS:77951844544

VL - 22

SP - 830

EP - 838

JO - Reproduction, Fertility and Development

JF - Reproduction, Fertility and Development

SN - 1031-3613

IS - 5

ER -