Meiotic progression, mitochondrial features and fertilisation characteristics of porcine oocytes with different G6PDH activities

István Egerszegi, Hannelore Alm, József Rátky, Bassiouni Heleil, Klaus Peter Brüssow, Helmut Torner

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31 Citations (Scopus)


The aim of the present study was to investigate the developmental competence, mitochondrial characteristics and chromatin status of immature follicular porcine oocytes selected for their glucose-6-phosphate dehydrogenase (G6PDH) activity by brilliant cresyl blue (BCB) staining. In Experiment 1, the oocyte parameters were determined in parallel right after BCB staining (T 0), after 22 h of in vitro maturation (IVM) (T22) and after 44 h of IVM (T44) (n=496). BCB-stained oocytes (BCB+) at T 0 were characterised by fibrillated chromatin filaments in their germinal vesicles (GV) and diakinesis stages whereas unstained (BCB-) oocytes at T0 contained in their GV mainly condensed stages of chromatin (P <0.05). After 22 h of IVM BCB+ oocytes showed a prominent chromatin configuration of metaphase I and after 44 h the majority developed a M II nuclear configuration in contrast to the BCB- group (P <0.0001). Differences were also observed between the two oocyte populations in their mitochondrial activity (P <0.05). At the beginning of IVM BCB+ oocytes were characterised by high mitochondrial activity in their cytoplasm. The BCB+ oocytes showed clear visible homogenous distributions of mitochondria (P <0.005) and contained more aggregated clusters of mitochondria in contrast to BCB- oocytes (P <0.005). In Experiment 2, 318 oocytes were tested for their G6PDH activity and introduced to IVM and IVF. Only oocytes from the BCB+ group, which were matured after 44 h up to the stage of M II (81.6%) were fertilised (17.4%), penetrated (46%) or activated (15.6%) after IVF. These results indicate a relationship between the G6PDH activity of porcine oocytes before IVM and their subsequent nuclear development, mitochondrial activity and aggregation.

Original languageEnglish
Pages (from-to)830-838
Number of pages9
JournalReproduction, Fertility and Development
Issue number5
Publication statusPublished - May 10 2010


  • Mitochondria
  • Pig

ASJC Scopus subject areas

  • Biotechnology
  • Reproductive Medicine
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology

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