Medullary adrenergic neurons contribute to the cocaine- and amphetamine-regulated transcript-immunoreactive innervation of thyrotropin-releasing hormone synthesizing neurons in the hypothalamic paraventricular nucleus

Gábor Wittmann, Z. Liposits, Ronald M. Lechan, Csaba Fekete

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Cocaine- and amphetamine-regulated transcript (CART)-IR axons densely innervate the thyrotropin-releasing hormone (TRH) neurons in the hypothalamic paraventricular nucleus (PVN), partly arising from neuronal perikarya in the hypothalamic arcuate nucleus. The source of the remaining CART innervation, however, is unknown. We have recently demonstrated that neurons co-containing adrenaline and CART in the C1-3 areas of the medulla project to the PVN. Since adrenergic neurons densely innervate the hypophysiotropic TRH neurons, we raised the possibility that adrenergic neurons contribute to the CART-IR innervation of hypophysiotropic TRH neurons. Combined in situ hybridization and immunocytochemistry was performed to study the colocalization of CART and phenylethanolamine N-methyltransferase (PNMT), the synthesizing enzyme of adrenaline, in axons innervating the hypophysiotropic TRH neurons. PNMT was observed in 44% of CART-IR axons in juxtaposition to the hypophysiotropic TRH neurons and CART-IR was observed in approximately 50% of all PNMT axons in contact with proTRH perikarya in the PVN. We conclude that adrenergic neurons of the medulla give rise to approximately half of the CART-IR axons innervating hypophysiotropic TRH neurons in the PVN, and propose that CART may play important role in the modulation of adrenergic input to the hypothalamic- pituitary-thyroid axis.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalBrain Research
Volume1006
Issue number1
DOIs
Publication statusPublished - Apr 23 2004

Fingerprint

Adrenergic Neurons
Thyrotropin-Releasing Hormone
Paraventricular Hypothalamic Nucleus
Amphetamine
Cocaine
Pituitary Hormone-Releasing Hormones
Neurons
Axons
Phenylethanolamine N-Methyltransferase
Epinephrine
Arcuate Nucleus of Hypothalamus
Adrenergic Agents
In Situ Hybridization
Thyroid Gland
Immunohistochemistry

Keywords

  • Adrenergic neuron
  • Cocaine- and amphetamine-regulated transcript
  • Colocalization
  • Hypothalamic paraventricular nucleus
  • Phenylethanolamine N-methyltransferase
  • Thyrotropin-releasing hormone

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

@article{8e90c523d4c34937b6f95be3fe0b7240,
title = "Medullary adrenergic neurons contribute to the cocaine- and amphetamine-regulated transcript-immunoreactive innervation of thyrotropin-releasing hormone synthesizing neurons in the hypothalamic paraventricular nucleus",
abstract = "Cocaine- and amphetamine-regulated transcript (CART)-IR axons densely innervate the thyrotropin-releasing hormone (TRH) neurons in the hypothalamic paraventricular nucleus (PVN), partly arising from neuronal perikarya in the hypothalamic arcuate nucleus. The source of the remaining CART innervation, however, is unknown. We have recently demonstrated that neurons co-containing adrenaline and CART in the C1-3 areas of the medulla project to the PVN. Since adrenergic neurons densely innervate the hypophysiotropic TRH neurons, we raised the possibility that adrenergic neurons contribute to the CART-IR innervation of hypophysiotropic TRH neurons. Combined in situ hybridization and immunocytochemistry was performed to study the colocalization of CART and phenylethanolamine N-methyltransferase (PNMT), the synthesizing enzyme of adrenaline, in axons innervating the hypophysiotropic TRH neurons. PNMT was observed in 44{\%} of CART-IR axons in juxtaposition to the hypophysiotropic TRH neurons and CART-IR was observed in approximately 50{\%} of all PNMT axons in contact with proTRH perikarya in the PVN. We conclude that adrenergic neurons of the medulla give rise to approximately half of the CART-IR axons innervating hypophysiotropic TRH neurons in the PVN, and propose that CART may play important role in the modulation of adrenergic input to the hypothalamic- pituitary-thyroid axis.",
keywords = "Adrenergic neuron, Cocaine- and amphetamine-regulated transcript, Colocalization, Hypothalamic paraventricular nucleus, Phenylethanolamine N-methyltransferase, Thyrotropin-releasing hormone",
author = "G{\'a}bor Wittmann and Z. Liposits and Lechan, {Ronald M.} and Csaba Fekete",
year = "2004",
month = "4",
day = "23",
doi = "10.1016/j.brainres.2003.12.049",
language = "English",
volume = "1006",
pages = "1--7",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Medullary adrenergic neurons contribute to the cocaine- and amphetamine-regulated transcript-immunoreactive innervation of thyrotropin-releasing hormone synthesizing neurons in the hypothalamic paraventricular nucleus

AU - Wittmann, Gábor

AU - Liposits, Z.

AU - Lechan, Ronald M.

AU - Fekete, Csaba

PY - 2004/4/23

Y1 - 2004/4/23

N2 - Cocaine- and amphetamine-regulated transcript (CART)-IR axons densely innervate the thyrotropin-releasing hormone (TRH) neurons in the hypothalamic paraventricular nucleus (PVN), partly arising from neuronal perikarya in the hypothalamic arcuate nucleus. The source of the remaining CART innervation, however, is unknown. We have recently demonstrated that neurons co-containing adrenaline and CART in the C1-3 areas of the medulla project to the PVN. Since adrenergic neurons densely innervate the hypophysiotropic TRH neurons, we raised the possibility that adrenergic neurons contribute to the CART-IR innervation of hypophysiotropic TRH neurons. Combined in situ hybridization and immunocytochemistry was performed to study the colocalization of CART and phenylethanolamine N-methyltransferase (PNMT), the synthesizing enzyme of adrenaline, in axons innervating the hypophysiotropic TRH neurons. PNMT was observed in 44% of CART-IR axons in juxtaposition to the hypophysiotropic TRH neurons and CART-IR was observed in approximately 50% of all PNMT axons in contact with proTRH perikarya in the PVN. We conclude that adrenergic neurons of the medulla give rise to approximately half of the CART-IR axons innervating hypophysiotropic TRH neurons in the PVN, and propose that CART may play important role in the modulation of adrenergic input to the hypothalamic- pituitary-thyroid axis.

AB - Cocaine- and amphetamine-regulated transcript (CART)-IR axons densely innervate the thyrotropin-releasing hormone (TRH) neurons in the hypothalamic paraventricular nucleus (PVN), partly arising from neuronal perikarya in the hypothalamic arcuate nucleus. The source of the remaining CART innervation, however, is unknown. We have recently demonstrated that neurons co-containing adrenaline and CART in the C1-3 areas of the medulla project to the PVN. Since adrenergic neurons densely innervate the hypophysiotropic TRH neurons, we raised the possibility that adrenergic neurons contribute to the CART-IR innervation of hypophysiotropic TRH neurons. Combined in situ hybridization and immunocytochemistry was performed to study the colocalization of CART and phenylethanolamine N-methyltransferase (PNMT), the synthesizing enzyme of adrenaline, in axons innervating the hypophysiotropic TRH neurons. PNMT was observed in 44% of CART-IR axons in juxtaposition to the hypophysiotropic TRH neurons and CART-IR was observed in approximately 50% of all PNMT axons in contact with proTRH perikarya in the PVN. We conclude that adrenergic neurons of the medulla give rise to approximately half of the CART-IR axons innervating hypophysiotropic TRH neurons in the PVN, and propose that CART may play important role in the modulation of adrenergic input to the hypothalamic- pituitary-thyroid axis.

KW - Adrenergic neuron

KW - Cocaine- and amphetamine-regulated transcript

KW - Colocalization

KW - Hypothalamic paraventricular nucleus

KW - Phenylethanolamine N-methyltransferase

KW - Thyrotropin-releasing hormone

UR - http://www.scopus.com/inward/record.url?scp=1642375950&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642375950&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2003.12.049

DO - 10.1016/j.brainres.2003.12.049

M3 - Article

VL - 1006

SP - 1

EP - 7

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -