The fluid phase of Tetrahymena cultures treated and not treated with insulin for 1 h stimulated the insulin binding of Chang liver cells to a similar degree as plain yeast extract containing Bacto tryptone medium. The fluid phase of mixed (1:1) cultures of insulin-imprinted and virgin Tetrahymena cells accounted for a significant increase in insulin binding relative to all other systems, and also to that of Chang liver cell cultures directly imprinted with insulin. It appears that the cell-cell transmission of hormonal information in Tetrahymena cultures is associated with release into the nutrient medium of a factor, which is able to transmit the information pertaining to imprinting to mammalian cells.
|Number of pages||5|
|Publication status||Published - Sep 26 1990|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)