Median raphe region stimulation alone generates remote, but not recent fear memory traces

Diána G. Balázsfi, Dóra Zelena, Lívia Farkas, Kornél Demeter, I. Barna, Csaba Cserép, Virág T. Takács, G. Nyíri, Flóra Gölöncsér, B. Sperlágh, T. Freund, József Haller

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The median raphe region (MRR) is believed to control the fear circuitry indirectly, by influencing the encoding and retrieval of fear memories by amygdala, hippocampus and prefrontal cortex. Here we show that in addition to this established role, MRR stimulation may alone elicit the emergence of remote but not recent fear memories. We substituted electric shocks with optic stimulation of MRR in C57BL/6N male mice in an optogenetic conditioning paradigm and found that stimulations produced agitation, but not fear, during the conditioning trial. Contextual fear, reflected by freezing was not present the next day, but appeared after a 7 days incubation. The optogenetic silencing of MRR during electric shocks ameliorated conditioned fear also seven, but not one day after conditioning. The optogenetic stimulation patterns (50Hz theta burst and 20Hz) used in our tests elicited serotonin release in vitro and lead to activation primarily in the periaqueductal gray examined by c-Fos immunohistochemistry. Earlier studies demonstrated that fear can be induced acutely by stimulation of several subcortical centers, which, however, do not generate persistent fear memories. Here we show that the MRR also elicits fear, but this develops slowly over time, likely by plastic changes induced by the area and its connections. These findings assign a specific role to the MRR in fear learning. Particularly, we suggest that this area is responsible for the durable sensitization of fear circuits towards aversive contexts, and by this, it contributes to the persistence of fear memories. This suggests the existence a bottom-up control of fear circuits by the MRR, which complements the top-down control exerted by the medial prefrontal cortex.

Original languageEnglish
Article numbere0181264
JournalPLoS One
Volume12
Issue number7
DOIs
Publication statusPublished - Jul 1 2017

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Balázsfi, D. G., Zelena, D., Farkas, L., Demeter, K., Barna, I., Cserép, C., Takács, V. T., Nyíri, G., Gölöncsér, F., Sperlágh, B., Freund, T., & Haller, J. (2017). Median raphe region stimulation alone generates remote, but not recent fear memory traces. PLoS One, 12(7), [e0181264]. https://doi.org/10.1371/journal.pone.0181264