Median eminence dopamine and serotonin neuronal activity: Temporal relationship to preovulatory prolactin and luteinizing hormone surges

Bernard Kerdelhué, Florence Bojda, Philippe Lesieur, Catherine Pasqualini, Amor El Abed, Véronique Lenoir, Patrice Douillet, Mike C. Chiueh, M. Palkóvits

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Using a high-performance liquid chromatography (HPLC) system coupled with an electrochemical detector, the concentrations of dopamine (DA) and 5-hydroxytryptamine (5- HT) and their major specific metabolites 3,4-dihydroxy-phenylaceticacid (DOPAC)and 5-hydroxyindole-3-acetic acid (5-HIAA), respectively, were measuredin the median eminence (ME) throughout the rat estrous cycle. The ME DA content remained fairly constant throughout the estrous cycle except on estrus when 17.00 h values were significantly lower than 10.00 h values (40% decrease, p <0.05). The ME 5-HT content determined at 10.00 h was higher on proestrus than on any other day of the cycle. The ME DOPAC concentrations did not differ between 10.00 and 17.00 h on diestrus I, diestrus II or estrus. On the contrary, there was an almost linear decline between 10.00 and 17.00 h on proestrus (36% decrease, p <0.05). The ME 5-FI IAA content did not differ between 10.00 and 17.00 h on any day of the estrous cycle. Significant changes were recorded for the DOPAC/DA and 5-HIAA 5-HT ratio in the ME on proestrus. There was a progressive decrease, starting from lO.OOh in the DOPAC/DA ratio with minimal values (42% decrease, p <0.05) at I6.00h followed by an increase from 16.00 to 19.00h. On the other hand, the 5-HIAA/5-HT ratio increased between 10.00 and 17.00h (97% increase, p <0.05) and subsequently declined until 19.00 h (67% decrease vs. 17.00 h. p <0.05). These data show that a concomitant decrease in dopaminergic activity and increase in serotoninergic activity in the ME occur in association with the preovulatory prolactin and luteinizing hormone preovulatory surges.

Original languageEnglish
Pages (from-to)176-180
Number of pages5
JournalNeuroendocrinology
Volume49
Issue number2
DOIs
Publication statusPublished - 1989

Fingerprint

Median Eminence
Luteinizing Hormone
Prolactin
Dopamine
Serotonin
Proestrus
Estrous Cycle
Acetic Acid
Diestrus
Estrus
High Pressure Liquid Chromatography
5-hydroxyindole

Keywords

  • Dopamine
  • Luteinizing hormone
  • Median eminence
  • Prolactin
  • Rat estrous cycle
  • Serotonin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Neuroscience(all)

Cite this

Median eminence dopamine and serotonin neuronal activity : Temporal relationship to preovulatory prolactin and luteinizing hormone surges. / Kerdelhué, Bernard; Bojda, Florence; Lesieur, Philippe; Pasqualini, Catherine; Abed, Amor El; Lenoir, Véronique; Douillet, Patrice; Chiueh, Mike C.; Palkóvits, M.

In: Neuroendocrinology, Vol. 49, No. 2, 1989, p. 176-180.

Research output: Contribution to journalArticle

Kerdelhué, B, Bojda, F, Lesieur, P, Pasqualini, C, Abed, AE, Lenoir, V, Douillet, P, Chiueh, MC & Palkóvits, M 1989, 'Median eminence dopamine and serotonin neuronal activity: Temporal relationship to preovulatory prolactin and luteinizing hormone surges', Neuroendocrinology, vol. 49, no. 2, pp. 176-180. https://doi.org/10.1159/000125111
Kerdelhué, Bernard ; Bojda, Florence ; Lesieur, Philippe ; Pasqualini, Catherine ; Abed, Amor El ; Lenoir, Véronique ; Douillet, Patrice ; Chiueh, Mike C. ; Palkóvits, M. / Median eminence dopamine and serotonin neuronal activity : Temporal relationship to preovulatory prolactin and luteinizing hormone surges. In: Neuroendocrinology. 1989 ; Vol. 49, No. 2. pp. 176-180.
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