Mechanisms and functions of AT1 angiotensin receptor internalization

László Hunyady, Kevin J. Catt, Adrian J.L. Clark, Zsuzsanna Gáborik

Research output: Contribution to journalReview article

91 Citations (Scopus)

Abstract

The type 1 (AT1) angiotensin receptor, which mediates the known physiological and pharmacological actions of angiotensin II, activates numerous intracellular signaling pathways and undergoes rapid internalization upon agonist binding. Morphological and biochemical studies have shown that agonist-induced endocytosis of the AT1 receptor occurs via clathrin-coated pits, and is dependent on two regions in the cytoplasmic tail of the receptor. However, it is independent of G protein activation and signaling, and does not require the conserved NPXXY motif in the seventh transmembrane helix. The dependence of internalization of the AT1 receptor on a cytoplasmic serine-threonine-rich region that is phosphorylated during agonist stimulation suggests that endocytosis is regulated by phosphorylation of the AT1 receptor tail. β-Arrestins have been implicated in the desensitization and endocytosis of several G protein-coupled receptors, but the exact nature of the adaptor protein required for association of the AT1 receptor with clathrin-coated pits, and the role of dynamin in the internalization process, are still controversial. There is increasing evidence for a role of internalization in sustained signal generation from the AT1 receptor. Several aspects of the mechanisms and specific function of AT1 receptor internalization, including its precise mode and route of endocytosis, and the potential roles of cytoplasmic and nuclear receptors, remain to be elucidated. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)29-44
Number of pages16
JournalRegulatory Peptides
Volume91
Issue number1-3
DOIs
Publication statusPublished - Jul 28 2000

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Keywords

  • Ca-mobilizing hormone
  • Dynamin
  • Endocytosis
  • G protein-coupled receptor
  • Phosphorylation
  • β-Arrestin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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