Mechanism of the positive inotropic effect of dimethyl sulfoxide at low doses in excised frog heart preparations

Ilyana V. Sazdova, Bilyana M. Ilieva, Milena Shkodrova, Rudolf Schubert, F. Fülöp, Hristo S. Gagov

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this study is to investigate the mechanism of the positive inotropic effect of repeated applications of 0.1% DMSO (3 times at 15 min intervals) on excised heart preparations of the frog Rana ridibunda. The positive inotropic effect of DMSO was abolished in the presence of prazosin, an alpha-adrenoceptor blocker, NCDC, a non-specific phospholipase C inhibitor and by non-selective cation channel blockers 2-APB (20 μmol/l) and SKF 96365 (30 μmol/l), but was insensitive to 30 μmol/l propranolol, a beta-adrenoceptor blocker, and 10 μmol/l GF 109203x, a PKC inhibitor. Inhibition of the Na+- Ca2+ exchanger by 30 μmol/l KB-R7943 or 0.5 μmol/l SEA0400 strongly suppressed the inuence of DMSO on maximal force of contraction of frog hearts. It is concluded that repeatedly applied 0.1% DMSO induces a positive inotropic effect in excised frog heart preparations mediated by a diacylglycerol-sensitive activation of transient receptor potential cation channels and partly by a stimulation of Ca2+ inux via the reverse mode of Na+-Ca2+ exchangers. Thus, low doses of DMSO have to be used cautiously in amphibians, as DMSO may change significantly the effect of dissolved substances by its own inuence.

Original languageEnglish
Pages (from-to)699-708
Number of pages10
JournalComptes Rendus de L'Academie Bulgare des Sciences
Volume63
Issue number5
Publication statusPublished - 2010

Fingerprint

Dimethyl Sulfoxide
Anura
1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
Adrenergic Receptors
Rana ridibunda
Transient Receptor Potential Channels
Prazosin
Diglycerides
Type C Phospholipases
Amphibians
Propranolol
Cations

Keywords

  • Diacylglycerol
  • Inotropic
  • Na-ca exchanger
  • Store-operated channels
  • Trpc channels

ASJC Scopus subject areas

  • General

Cite this

Mechanism of the positive inotropic effect of dimethyl sulfoxide at low doses in excised frog heart preparations. / Sazdova, Ilyana V.; Ilieva, Bilyana M.; Shkodrova, Milena; Schubert, Rudolf; Fülöp, F.; Gagov, Hristo S.

In: Comptes Rendus de L'Academie Bulgare des Sciences, Vol. 63, No. 5, 2010, p. 699-708.

Research output: Contribution to journalArticle

Sazdova, Ilyana V. ; Ilieva, Bilyana M. ; Shkodrova, Milena ; Schubert, Rudolf ; Fülöp, F. ; Gagov, Hristo S. / Mechanism of the positive inotropic effect of dimethyl sulfoxide at low doses in excised frog heart preparations. In: Comptes Rendus de L'Academie Bulgare des Sciences. 2010 ; Vol. 63, No. 5. pp. 699-708.
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AU - Ilieva, Bilyana M.

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AU - Schubert, Rudolf

AU - Fülöp, F.

AU - Gagov, Hristo S.

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