Mechanism of the positive effect of poly(ethylene glycol) addition in enzymatic hydrolysis of steam pretreated lignocelluloses

Bálint Sipos, Mátyás Szilágyi, Zoltán Sebestyén, Raffaella Perazzini, Dóra Dienes, E. Jakab, Claudia Crestini, K. Réczey

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The efficiency of enzymatic hydrolysis of lignocellulses can be increased by addition of surfactants and polymers, such as poly(ethylene glycol) (PEG). The effect of PEG addition on the cellulase adsorption was tested on various steam pretreated lignocellulose substrates (spruce, willow, hemp, corn stover, wheat straw, sweet sorghum bagasse). A positive effect of PEG addition was observed, as protein adsorption has decreased and free enzyme activities (FP, β-glucosidase) have increased due to the additive. However, the degree of enhancement differed among the substrates, being highest on steam pretreated spruce. Results of lignin analysis (pyrolysis-GC/MS, 31P NMR) suggest that the effect of PEG addition is in connection with the amount of unsubstituted phenolic hydroxyl groups of lignin in the substrate. Adsorption experiments using two commercial enzyme preparations, Celluclast 1.5L (Trichoderma reesei cellulase) and Novozym 188 (Aspergillus niger β-glucosidase) suggested that enzyme origins affected on the adsorptivity of β-glucosidases.

Original languageEnglish
Pages (from-to)812-823
Number of pages12
JournalComptes Rendus - Biologies
Volume334
Issue number11
DOIs
Publication statusPublished - Nov 2011

Fingerprint

Glucosidases
lignocellulose
glucosidases
Enzymatic hydrolysis
Ethylene Glycol
Steam
enzymatic hydrolysis
polyethylene glycol
steam
Polyethylene glycols
Adsorption
adsorption
Hydrolysis
Cellulase
Lignin
endo-1,4-beta-glucanase
Picea
lignin
Enzymes
Salix

Keywords

  • β-glucosidase
  • Cellulase
  • Enzyme adsorption
  • Lignin
  • Lignocellulose
  • Poly(ethylene glycol)
  • Steam pretreatment

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Mechanism of the positive effect of poly(ethylene glycol) addition in enzymatic hydrolysis of steam pretreated lignocelluloses. / Sipos, Bálint; Szilágyi, Mátyás; Sebestyén, Zoltán; Perazzini, Raffaella; Dienes, Dóra; Jakab, E.; Crestini, Claudia; Réczey, K.

In: Comptes Rendus - Biologies, Vol. 334, No. 11, 11.2011, p. 812-823.

Research output: Contribution to journalArticle

Sipos, Bálint ; Szilágyi, Mátyás ; Sebestyén, Zoltán ; Perazzini, Raffaella ; Dienes, Dóra ; Jakab, E. ; Crestini, Claudia ; Réczey, K. / Mechanism of the positive effect of poly(ethylene glycol) addition in enzymatic hydrolysis of steam pretreated lignocelluloses. In: Comptes Rendus - Biologies. 2011 ; Vol. 334, No. 11. pp. 812-823.
@article{388dc77e1d644e25afa16b16a2d10949,
title = "Mechanism of the positive effect of poly(ethylene glycol) addition in enzymatic hydrolysis of steam pretreated lignocelluloses",
abstract = "The efficiency of enzymatic hydrolysis of lignocellulses can be increased by addition of surfactants and polymers, such as poly(ethylene glycol) (PEG). The effect of PEG addition on the cellulase adsorption was tested on various steam pretreated lignocellulose substrates (spruce, willow, hemp, corn stover, wheat straw, sweet sorghum bagasse). A positive effect of PEG addition was observed, as protein adsorption has decreased and free enzyme activities (FP, β-glucosidase) have increased due to the additive. However, the degree of enhancement differed among the substrates, being highest on steam pretreated spruce. Results of lignin analysis (pyrolysis-GC/MS, 31P NMR) suggest that the effect of PEG addition is in connection with the amount of unsubstituted phenolic hydroxyl groups of lignin in the substrate. Adsorption experiments using two commercial enzyme preparations, Celluclast 1.5L (Trichoderma reesei cellulase) and Novozym 188 (Aspergillus niger β-glucosidase) suggested that enzyme origins affected on the adsorptivity of β-glucosidases.",
keywords = "β-glucosidase, Cellulase, Enzyme adsorption, Lignin, Lignocellulose, Poly(ethylene glycol), Steam pretreatment",
author = "B{\'a}lint Sipos and M{\'a}ty{\'a}s Szil{\'a}gyi and Zolt{\'a}n Sebesty{\'e}n and Raffaella Perazzini and D{\'o}ra Dienes and E. Jakab and Claudia Crestini and K. R{\'e}czey",
year = "2011",
month = "11",
doi = "10.1016/j.crvi.2011.06.005",
language = "English",
volume = "334",
pages = "812--823",
journal = "Comptes Rendus - Biologies",
issn = "1631-0691",
publisher = "Elsevier Masson",
number = "11",

}

TY - JOUR

T1 - Mechanism of the positive effect of poly(ethylene glycol) addition in enzymatic hydrolysis of steam pretreated lignocelluloses

AU - Sipos, Bálint

AU - Szilágyi, Mátyás

AU - Sebestyén, Zoltán

AU - Perazzini, Raffaella

AU - Dienes, Dóra

AU - Jakab, E.

AU - Crestini, Claudia

AU - Réczey, K.

PY - 2011/11

Y1 - 2011/11

N2 - The efficiency of enzymatic hydrolysis of lignocellulses can be increased by addition of surfactants and polymers, such as poly(ethylene glycol) (PEG). The effect of PEG addition on the cellulase adsorption was tested on various steam pretreated lignocellulose substrates (spruce, willow, hemp, corn stover, wheat straw, sweet sorghum bagasse). A positive effect of PEG addition was observed, as protein adsorption has decreased and free enzyme activities (FP, β-glucosidase) have increased due to the additive. However, the degree of enhancement differed among the substrates, being highest on steam pretreated spruce. Results of lignin analysis (pyrolysis-GC/MS, 31P NMR) suggest that the effect of PEG addition is in connection with the amount of unsubstituted phenolic hydroxyl groups of lignin in the substrate. Adsorption experiments using two commercial enzyme preparations, Celluclast 1.5L (Trichoderma reesei cellulase) and Novozym 188 (Aspergillus niger β-glucosidase) suggested that enzyme origins affected on the adsorptivity of β-glucosidases.

AB - The efficiency of enzymatic hydrolysis of lignocellulses can be increased by addition of surfactants and polymers, such as poly(ethylene glycol) (PEG). The effect of PEG addition on the cellulase adsorption was tested on various steam pretreated lignocellulose substrates (spruce, willow, hemp, corn stover, wheat straw, sweet sorghum bagasse). A positive effect of PEG addition was observed, as protein adsorption has decreased and free enzyme activities (FP, β-glucosidase) have increased due to the additive. However, the degree of enhancement differed among the substrates, being highest on steam pretreated spruce. Results of lignin analysis (pyrolysis-GC/MS, 31P NMR) suggest that the effect of PEG addition is in connection with the amount of unsubstituted phenolic hydroxyl groups of lignin in the substrate. Adsorption experiments using two commercial enzyme preparations, Celluclast 1.5L (Trichoderma reesei cellulase) and Novozym 188 (Aspergillus niger β-glucosidase) suggested that enzyme origins affected on the adsorptivity of β-glucosidases.

KW - β-glucosidase

KW - Cellulase

KW - Enzyme adsorption

KW - Lignin

KW - Lignocellulose

KW - Poly(ethylene glycol)

KW - Steam pretreatment

UR - http://www.scopus.com/inward/record.url?scp=80955163665&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80955163665&partnerID=8YFLogxK

U2 - 10.1016/j.crvi.2011.06.005

DO - 10.1016/j.crvi.2011.06.005

M3 - Article

C2 - 22078738

AN - SCOPUS:80955163665

VL - 334

SP - 812

EP - 823

JO - Comptes Rendus - Biologies

JF - Comptes Rendus - Biologies

SN - 1631-0691

IS - 11

ER -