Mechanism of glucocorticoid-induced depletion of human CD14 +CD16+ monocytes

Farshid Dayyani, Kai Uwe Beige, Marion Frankenberger, Matthias Mack, T. Berki, Loems Ziegler-Heitbrock

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Healthy donors infused with high doses of glucocorticoids [GCs; methyl-prednisolone (MP); 500 mg/day for 3 days] suffer a selective depletion of the CD14+CD16+ monocytes such that these cells are reduced by 95% on day 5. In vitro studies revealed that at 11 h of culture in the presence of 10-5 M MP, no depletion was observed as yet, but a reduction by 80% was seen after 24 h. In dose-response analysis, MP still led to a 50% reduction of CD14+CD16+ monocytes at 10 -7 M. Depletion could not be overcome by addition of the cytokines interleukin-1β or macrophage-colony stimulating factor, and it was independent of CD95. Depletion was, however, inhibited by the caspase 3,8 blocker z-Val-Ala-Asp, suggesting that cell death occurs in a caspase-dependent manner. Furthermore, blockade of depletion by RU-486 indicates that the intracellular GC receptor (GCR) is involved. Measurement of GCR by flow cytometry revealed a 50% higher level of expression in the CD14 +CD16+ monocytes. Our studies show a selective depletion of CD14+CD16+ monocytes by GC treatment in vivo and in vitro, an effect to which the modestly increased level of GCR may contribute.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalJournal of Leukocyte Biology
Volume74
Issue number1
DOIs
Publication statusPublished - Jul 2003

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Glucocorticoids
Monocytes
Prednisolone
Mifepristone
Macrophage Colony-Stimulating Factor
Caspase 8
Caspases
Interleukin-1
Caspase 3
Flow Cytometry
Cell Death
Cytokines
In Vitro Techniques

Keywords

  • Apoptosis
  • Glucocorticoids
  • Macrophage
  • Monocyte

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dayyani, F., Beige, K. U., Frankenberger, M., Mack, M., Berki, T., & Ziegler-Heitbrock, L. (2003). Mechanism of glucocorticoid-induced depletion of human CD14 +CD16+ monocytes. Journal of Leukocyte Biology, 74(1), 33-39. https://doi.org/10.1189/jlb.1202612

Mechanism of glucocorticoid-induced depletion of human CD14 +CD16+ monocytes. / Dayyani, Farshid; Beige, Kai Uwe; Frankenberger, Marion; Mack, Matthias; Berki, T.; Ziegler-Heitbrock, Loems.

In: Journal of Leukocyte Biology, Vol. 74, No. 1, 07.2003, p. 33-39.

Research output: Contribution to journalArticle

Dayyani, F, Beige, KU, Frankenberger, M, Mack, M, Berki, T & Ziegler-Heitbrock, L 2003, 'Mechanism of glucocorticoid-induced depletion of human CD14 +CD16+ monocytes', Journal of Leukocyte Biology, vol. 74, no. 1, pp. 33-39. https://doi.org/10.1189/jlb.1202612
Dayyani, Farshid ; Beige, Kai Uwe ; Frankenberger, Marion ; Mack, Matthias ; Berki, T. ; Ziegler-Heitbrock, Loems. / Mechanism of glucocorticoid-induced depletion of human CD14 +CD16+ monocytes. In: Journal of Leukocyte Biology. 2003 ; Vol. 74, No. 1. pp. 33-39.
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