Mechanism of generation and regulation of photopotential by bacteriorhodopsin in bimolecular lipid membrane. The quenching effect of blue light

P. Ormos, Zs Dancsházy, B. Karvaly

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Photoelectric properties of bacteriorhodopsin incorporated into a bimolecular lipid membrane were investigated with special regard to the mechanism of photoelectric field generation. It was shown that besides its proton pump and electric generator functions bacteriorhodopsin works as a possible molecular regulator of the light-induced membrane potential. When a bimolecular lipid membrane containing bacteriorhodopsin is continuously illuminated in its main visible absorption band, and afterwards by superimposed blue light matching the absorption band of the long-living photobleached bacteriorhodopsin (M412) as well, the latter either enhances or decreases the steady-state photoresponse, depending upon the intensity of the green light. Thus, the additional blue-light illumination tends to cause the resultant photoelectric membrane potential to become stabilized. Two alternative schemes are tentatively proposed for the photochemical cycle of bacteriorhodopsin whereby blue light can control photovoltage generation. A kinetic model of the proton pump and the regulation of the photoelectric membrane potential is presented. This model fits all the experimental findings, even quantitatively. From the model some kinetic and physical parameters of this light-driven pump could be determined.

Original languageEnglish
Pages (from-to)304-315
Number of pages12
JournalBBA - Bioenergetics
Volume503
Issue number2
DOIs
Publication statusPublished - Aug 8 1978

Fingerprint

Bacteriorhodopsins
Membrane Lipids
Quenching
Light
Membrane Potentials
Proton Pumps
Membranes
Absorption spectra
Electric generators
Methyl Green
Kinetics
Lighting
Pumps

ASJC Scopus subject areas

  • Biophysics
  • Medicine(all)

Cite this

Mechanism of generation and regulation of photopotential by bacteriorhodopsin in bimolecular lipid membrane. The quenching effect of blue light. / Ormos, P.; Dancsházy, Zs; Karvaly, B.

In: BBA - Bioenergetics, Vol. 503, No. 2, 08.08.1978, p. 304-315.

Research output: Contribution to journalArticle

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