Mechanism and consequences of the shift in cardiac arginine metabolism following ischaemia and reperfusion in rats

Rolf Schreckenberg, Pia Weber, Hector A. Cabrera-Fuentes, Isabel Steinert, Klaus T. Preissner, Péter Bencsik, Márta Sárközy, C. Csonka, P. Ferdinándy, Rainer Schulz, Klaus Dieter Schlüter

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Cardiac ischaemia and reperfusion leads to irreversible injury and subsequent tissue remodelling. Initial reperfusion seems to shift arginine metabolism from nitric oxide (NO) to polyamine formation. This may limit functional recovery at reperfusion. The hypothesis was tested whether ischaemia/reperfusion translates such a shift in arginine metabolism in a tumour necrosis factor (TNF)-α-dependent way and renin-angiotensin system (RAS)-dependent way into a sustained effect. Both, the early post-ischaemic recovery and molecular adaptation to ischaemia/reperfusion were analysed in saline perfused rat hearts undergoing global no-flow ischaemia and reperfusion. Local TNF-α activation was blocked by inhibition of TNF-α sheddase ADAM17. To interfere with RAS captopril was administered. Arginase was inhibited by administration of Nor-NOHA. Long-term effects of ischemia/reperfusion on arginine metabolism were analysed in vivo in rats receiving an established ischaemia/reperfusion protocol in the closed chest mode. mRNA expression analysis indicated a shift in the arginine metabolism from NO formation to polyamine metabolism starting within 2 hours (h) of reperfusion and translated into protein expression within 24 h. Inhibition of the TNF-α pathway and captopril attenuated these delayed effects on post-ischaemic recovery. This shift in arginine metabolism was associated with functional impairment of hearts within 24 h. Inhibition of arginase but not that of TNF-α and RAS pathways improved functional recovery immediately. However, no benefit was observed after four months. In conclusion, this study identified TNF-α and RAS to be responsible for depressed cardiac function that occurred a few hours after reperfusion.

Original languageEnglish
Pages (from-to)482-493
Number of pages12
JournalThrombosis and Haemostasis
Volume113
Issue number3
DOIs
Publication statusPublished - 2015

Fingerprint

Reperfusion
Arginine
Ischemia
Tumor Necrosis Factor-alpha
Renin-Angiotensin System
Arginase
Captopril
Polyamines
Nitric Oxide
Thorax
Messenger RNA
Wounds and Injuries

ASJC Scopus subject areas

  • Hematology

Cite this

Schreckenberg, R., Weber, P., Cabrera-Fuentes, H. A., Steinert, I., Preissner, K. T., Bencsik, P., ... Schlüter, K. D. (2015). Mechanism and consequences of the shift in cardiac arginine metabolism following ischaemia and reperfusion in rats. Thrombosis and Haemostasis, 113(3), 482-493. https://doi.org/10.1160/TH14-05-0477

Mechanism and consequences of the shift in cardiac arginine metabolism following ischaemia and reperfusion in rats. / Schreckenberg, Rolf; Weber, Pia; Cabrera-Fuentes, Hector A.; Steinert, Isabel; Preissner, Klaus T.; Bencsik, Péter; Sárközy, Márta; Csonka, C.; Ferdinándy, P.; Schulz, Rainer; Schlüter, Klaus Dieter.

In: Thrombosis and Haemostasis, Vol. 113, No. 3, 2015, p. 482-493.

Research output: Contribution to journalArticle

Schreckenberg, R, Weber, P, Cabrera-Fuentes, HA, Steinert, I, Preissner, KT, Bencsik, P, Sárközy, M, Csonka, C, Ferdinándy, P, Schulz, R & Schlüter, KD 2015, 'Mechanism and consequences of the shift in cardiac arginine metabolism following ischaemia and reperfusion in rats', Thrombosis and Haemostasis, vol. 113, no. 3, pp. 482-493. https://doi.org/10.1160/TH14-05-0477
Schreckenberg, Rolf ; Weber, Pia ; Cabrera-Fuentes, Hector A. ; Steinert, Isabel ; Preissner, Klaus T. ; Bencsik, Péter ; Sárközy, Márta ; Csonka, C. ; Ferdinándy, P. ; Schulz, Rainer ; Schlüter, Klaus Dieter. / Mechanism and consequences of the shift in cardiac arginine metabolism following ischaemia and reperfusion in rats. In: Thrombosis and Haemostasis. 2015 ; Vol. 113, No. 3. pp. 482-493.
@article{61e0df31aa964e0ba80ae0da5ce7bdfd,
title = "Mechanism and consequences of the shift in cardiac arginine metabolism following ischaemia and reperfusion in rats",
abstract = "Cardiac ischaemia and reperfusion leads to irreversible injury and subsequent tissue remodelling. Initial reperfusion seems to shift arginine metabolism from nitric oxide (NO) to polyamine formation. This may limit functional recovery at reperfusion. The hypothesis was tested whether ischaemia/reperfusion translates such a shift in arginine metabolism in a tumour necrosis factor (TNF)-α-dependent way and renin-angiotensin system (RAS)-dependent way into a sustained effect. Both, the early post-ischaemic recovery and molecular adaptation to ischaemia/reperfusion were analysed in saline perfused rat hearts undergoing global no-flow ischaemia and reperfusion. Local TNF-α activation was blocked by inhibition of TNF-α sheddase ADAM17. To interfere with RAS captopril was administered. Arginase was inhibited by administration of Nor-NOHA. Long-term effects of ischemia/reperfusion on arginine metabolism were analysed in vivo in rats receiving an established ischaemia/reperfusion protocol in the closed chest mode. mRNA expression analysis indicated a shift in the arginine metabolism from NO formation to polyamine metabolism starting within 2 hours (h) of reperfusion and translated into protein expression within 24 h. Inhibition of the TNF-α pathway and captopril attenuated these delayed effects on post-ischaemic recovery. This shift in arginine metabolism was associated with functional impairment of hearts within 24 h. Inhibition of arginase but not that of TNF-α and RAS pathways improved functional recovery immediately. However, no benefit was observed after four months. In conclusion, this study identified TNF-α and RAS to be responsible for depressed cardiac function that occurred a few hours after reperfusion.",
author = "Rolf Schreckenberg and Pia Weber and Cabrera-Fuentes, {Hector A.} and Isabel Steinert and Preissner, {Klaus T.} and P{\'e}ter Bencsik and M{\'a}rta S{\'a}rk{\"o}zy and C. Csonka and P. Ferdin{\'a}ndy and Rainer Schulz and Schl{\"u}ter, {Klaus Dieter}",
year = "2015",
doi = "10.1160/TH14-05-0477",
language = "English",
volume = "113",
pages = "482--493",
journal = "Thrombosis and Haemostasis",
issn = "0340-6245",
publisher = "Schattauer GmbH",
number = "3",

}

TY - JOUR

T1 - Mechanism and consequences of the shift in cardiac arginine metabolism following ischaemia and reperfusion in rats

AU - Schreckenberg, Rolf

AU - Weber, Pia

AU - Cabrera-Fuentes, Hector A.

AU - Steinert, Isabel

AU - Preissner, Klaus T.

AU - Bencsik, Péter

AU - Sárközy, Márta

AU - Csonka, C.

AU - Ferdinándy, P.

AU - Schulz, Rainer

AU - Schlüter, Klaus Dieter

PY - 2015

Y1 - 2015

N2 - Cardiac ischaemia and reperfusion leads to irreversible injury and subsequent tissue remodelling. Initial reperfusion seems to shift arginine metabolism from nitric oxide (NO) to polyamine formation. This may limit functional recovery at reperfusion. The hypothesis was tested whether ischaemia/reperfusion translates such a shift in arginine metabolism in a tumour necrosis factor (TNF)-α-dependent way and renin-angiotensin system (RAS)-dependent way into a sustained effect. Both, the early post-ischaemic recovery and molecular adaptation to ischaemia/reperfusion were analysed in saline perfused rat hearts undergoing global no-flow ischaemia and reperfusion. Local TNF-α activation was blocked by inhibition of TNF-α sheddase ADAM17. To interfere with RAS captopril was administered. Arginase was inhibited by administration of Nor-NOHA. Long-term effects of ischemia/reperfusion on arginine metabolism were analysed in vivo in rats receiving an established ischaemia/reperfusion protocol in the closed chest mode. mRNA expression analysis indicated a shift in the arginine metabolism from NO formation to polyamine metabolism starting within 2 hours (h) of reperfusion and translated into protein expression within 24 h. Inhibition of the TNF-α pathway and captopril attenuated these delayed effects on post-ischaemic recovery. This shift in arginine metabolism was associated with functional impairment of hearts within 24 h. Inhibition of arginase but not that of TNF-α and RAS pathways improved functional recovery immediately. However, no benefit was observed after four months. In conclusion, this study identified TNF-α and RAS to be responsible for depressed cardiac function that occurred a few hours after reperfusion.

AB - Cardiac ischaemia and reperfusion leads to irreversible injury and subsequent tissue remodelling. Initial reperfusion seems to shift arginine metabolism from nitric oxide (NO) to polyamine formation. This may limit functional recovery at reperfusion. The hypothesis was tested whether ischaemia/reperfusion translates such a shift in arginine metabolism in a tumour necrosis factor (TNF)-α-dependent way and renin-angiotensin system (RAS)-dependent way into a sustained effect. Both, the early post-ischaemic recovery and molecular adaptation to ischaemia/reperfusion were analysed in saline perfused rat hearts undergoing global no-flow ischaemia and reperfusion. Local TNF-α activation was blocked by inhibition of TNF-α sheddase ADAM17. To interfere with RAS captopril was administered. Arginase was inhibited by administration of Nor-NOHA. Long-term effects of ischemia/reperfusion on arginine metabolism were analysed in vivo in rats receiving an established ischaemia/reperfusion protocol in the closed chest mode. mRNA expression analysis indicated a shift in the arginine metabolism from NO formation to polyamine metabolism starting within 2 hours (h) of reperfusion and translated into protein expression within 24 h. Inhibition of the TNF-α pathway and captopril attenuated these delayed effects on post-ischaemic recovery. This shift in arginine metabolism was associated with functional impairment of hearts within 24 h. Inhibition of arginase but not that of TNF-α and RAS pathways improved functional recovery immediately. However, no benefit was observed after four months. In conclusion, this study identified TNF-α and RAS to be responsible for depressed cardiac function that occurred a few hours after reperfusion.

UR - http://www.scopus.com/inward/record.url?scp=84924106106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924106106&partnerID=8YFLogxK

U2 - 10.1160/TH14-05-0477

DO - 10.1160/TH14-05-0477

M3 - Article

C2 - 25502809

AN - SCOPUS:84924106106

VL - 113

SP - 482

EP - 493

JO - Thrombosis and Haemostasis

JF - Thrombosis and Haemostasis

SN - 0340-6245

IS - 3

ER -