Mechanical stress alters protein O-GlcNAc in human periodontal ligament cells

Dorottya Frank, Annamária Cser, Béla Kolarovszki, Nelli Farkas, A. Miseta, T. Nagy

Research output: Contribution to journalArticle

Abstract

Protein O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of intracellular proteins that regulates several physiological and pathophysiological process, including response to various stressors. However, O-GlcNAc's response to mechanical stress has not been investigated yet. As human periodontal ligament (PDL) cells are stimulated by compression force during orthodontic tooth movement that results in structural remodelling, in this study we investigated whether mechanical stress induces any alteration in protein O-GlcNAc in PDL cells. In this study, PDL cells isolated from premolars extracted for orthodontic indications were exposed to 0, 1.5, 3, 7 and 14 g/cm2 compression forces for 12 hours. Cell viability was measured by flow cytometry, and protein O-GlcNAc was analysed by Western blot. Cellular structure and intracellular distribution of O-GlcNAc was studied by immunofluorescence microscopy. We found that between 1.5 and 3 g/cm2 mechanical compression, O-GlcNAc significantly elevated; however, at higher forces O-GlcNAc level was not increased. We also found that intracellular localization of O-GlcNAc proteins became more centralized under 2 g/cm2 compression force. Our results suggest that structural changes stimulated by compression forces have a significant effect on the regulation of O-GlcNAc; thus, it might play a role in the mechanical stress adaptation of PDL cells.

Original languageEnglish
Pages (from-to)6251-6259
Number of pages9
JournalJournal of Cellular and Molecular Medicine
Volume23
Issue number9
DOIs
Publication statusPublished - Jan 1 2019

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Tooth Movement Techniques
Periodontal Ligament
Mechanical Stress
Post Translational Protein Processing
Heat-Shock Proteins
Molecular Medicine
Proteins
Physiological Phenomena
Acetylglucosamine
Bicuspid
Cellular Structures
Orthodontics
Fluorescence Microscopy
Cell Survival
Flow Cytometry
Western Blotting

Keywords

  • O-GlcNAc
  • orthodontic tooth movement
  • Periodontal ligament cells
  • post-translational modifications
  • stress response

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology

Cite this

Mechanical stress alters protein O-GlcNAc in human periodontal ligament cells. / Frank, Dorottya; Cser, Annamária; Kolarovszki, Béla; Farkas, Nelli; Miseta, A.; Nagy, T.

In: Journal of Cellular and Molecular Medicine, Vol. 23, No. 9, 01.01.2019, p. 6251-6259.

Research output: Contribution to journalArticle

Frank, Dorottya ; Cser, Annamária ; Kolarovszki, Béla ; Farkas, Nelli ; Miseta, A. ; Nagy, T. / Mechanical stress alters protein O-GlcNAc in human periodontal ligament cells. In: Journal of Cellular and Molecular Medicine. 2019 ; Vol. 23, No. 9. pp. 6251-6259.
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