Measurements of myofibrillar protein breakdown in newborn human infants

J. L. Burgoyne, F. J. Ballard, F. M. Tomas, A. Dobozy, A. H. MacLennan, A. Fitzgerald, G. W. Dahlenburg

Research output: Contribution to journalArticle

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Abstract

Myofibrillar protein breakdown was calculated from the urinary excretion ratio of N(τ)-methylhistidine (3-methylhistidine) to creatinine in newborn premature and full-term infants. Representative values were obtained from single voidings provided that the infant's metabolic status was stable. N(τ)-methylhistidine in infant urine was measured by a rapid Auto Analyser method and shown to give similar values to those obtained by ion-exchange separation techniques. The molar excretion ratio of N(τ)-methylhistidine to creatinine avaraged 0.0159 in urine samples obtained within 12 h after birth. A similar ratio was found in amniotic fluid collected at birth. It is argued that this ratio does not reflect a low rate of myofibrillar protein breakdown in the foetus, but rather a more effective transplacental passage of N(τ)-methylhistidine than of creatinine. The urinary ratio increased during the first 2 days after birth to a plateau at 0.0372. This represents a myofibrillar protein degradation rate of 3.40% day -1 in full-term infants. The molar excretion ratio during the period 40-120 h after birth increased in premature infants and reflects a fractional degradation rate of 5.34% day -1 in those infants weighing less than 1 kg at birth. Lower excretion ratios were found in some infants of diabetic mothers and in athyroid infants. The urinary excretion ratio of N(τ)-methylhistidine to creatinine is presented as a useful method for evaluating the breakdown rate of myofibrillar protein in neonates and can be applied to a number of abnormal nutritional or hormonal states.

Original languageEnglish
Pages (from-to)421-427
Number of pages7
JournalClinical Science
Volume63
Issue number5
Publication statusPublished - 1982

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Methylhistidines
Newborn Infant
Parturition
Creatinine
Proteins
Urine
Ion Exchange
Amniotic Fluid
Premature Infants
Proteolysis
Fetus
Mothers

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Burgoyne, J. L., Ballard, F. J., Tomas, F. M., Dobozy, A., MacLennan, A. H., Fitzgerald, A., & Dahlenburg, G. W. (1982). Measurements of myofibrillar protein breakdown in newborn human infants. Clinical Science, 63(5), 421-427.

Measurements of myofibrillar protein breakdown in newborn human infants. / Burgoyne, J. L.; Ballard, F. J.; Tomas, F. M.; Dobozy, A.; MacLennan, A. H.; Fitzgerald, A.; Dahlenburg, G. W.

In: Clinical Science, Vol. 63, No. 5, 1982, p. 421-427.

Research output: Contribution to journalArticle

Burgoyne, JL, Ballard, FJ, Tomas, FM, Dobozy, A, MacLennan, AH, Fitzgerald, A & Dahlenburg, GW 1982, 'Measurements of myofibrillar protein breakdown in newborn human infants', Clinical Science, vol. 63, no. 5, pp. 421-427.
Burgoyne JL, Ballard FJ, Tomas FM, Dobozy A, MacLennan AH, Fitzgerald A et al. Measurements of myofibrillar protein breakdown in newborn human infants. Clinical Science. 1982;63(5):421-427.
Burgoyne, J. L. ; Ballard, F. J. ; Tomas, F. M. ; Dobozy, A. ; MacLennan, A. H. ; Fitzgerald, A. ; Dahlenburg, G. W. / Measurements of myofibrillar protein breakdown in newborn human infants. In: Clinical Science. 1982 ; Vol. 63, No. 5. pp. 421-427.
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