Measurement of the optical parameters of purple membrane and plant light-harvesting complex films with optical waveguide lightmode spectroscopy

A. Lukács, G. Garab, E. Papp

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purple membrane (bacteriorhodopsin) and plant light-harvesting complexes (LHCII) were dried on the optical waveguide sensor with varying thicknesses in a wide range (from 20 to several hundreds of nanometers) and the optical parameters were studied with optical waveguide lightmode spectroscopy. It was found that applying the approximate 4-layer mode equations for the measured effective refractive indices resulted in unacceptable results for the optical parameters: with increasing thickness the refractive index decreased monotonously from 1.5 to 1.1. Therefore an inverse waveguide numerical method was developed and used to obtain reliable results from the experiments. The inverse method yielded an approximately constant (1.53) refractive index independently of the thickness for the purple membrane and LHCII films. Light-induced changes in the optical parameters of the purple membrane and LHCII films were also studied. For purple membrane films the most significant effect is the change in refractive index and absorption. For LHCII films prolonged illumination induced irreversible structural changes, most probably of thermo-optic origin.

Original languageEnglish
Pages (from-to)1606-1612
Number of pages7
JournalBiosensors and Bioelectronics
Volume21
Issue number8
DOIs
Publication statusPublished - Feb 15 2006

Fingerprint

Purple Membrane
Refractometry
Optical waveguides
Refractive index
Spectrum Analysis
Spectroscopy
Membranes
Light
Bacteriorhodopsins
Motion Pictures
Lighting
Optics
Numerical methods
Waveguides
Sensors
Experiments

Keywords

  • LHCII
  • Mode equation
  • Optical waveguide lightmode spectroscopy
  • Purple membrane

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Measurement of the optical parameters of purple membrane and plant light-harvesting complex films with optical waveguide lightmode spectroscopy. / Lukács, A.; Garab, G.; Papp, E.

In: Biosensors and Bioelectronics, Vol. 21, No. 8, 15.02.2006, p. 1606-1612.

Research output: Contribution to journalArticle

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