Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in lacaune sheep

B. El Amiri, A. Karen, J. Sulon, N. Melo De Sousa, A. V. Alvarez-Oxiley, Y. Cognié, O. Szenci, J. F. Beckers

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This study describes ovine pregnancy-associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi-purified ovPAG as standard, tracer and immunogens for antibody production in rabbits. Antisera R780 (against ovPAG57+59kDa) and R805 (against ovPAG558+61kDa) were used respectively in RIA-780 and RIA-805. Blood samples were collected at days 0, 18, 20, 22 and 25 after artificial insemination. From day 18 after breeding onward, the mean ovPAG concentration was significantly higher (p <0.001) in plasma samples from pregnant ewes (n = 17) than in non-pregnant ones (n = 3). The specific activity of the tracer was 11 760 Ci/mmol in RIA-780 and 14 900 Ci/mmol in RIA-805. The minimal detection limits for RIA-780 and RIA-805 were 0.2 ng/ml and 0.3 ng/ml, respectively. The intra-assay CV of samples with low (1.0 ng/ml), medium (2.5 ng/ml) and high (4.0 ng/ml) PAG concentrations were 3%, 6% and 9% for RIA-780 and 8%, 9% and 5% for RIA-805. The inter-assay CV in the same samples were 13%, 12% and 7% for RIA-780 and 13%, 11% and 5% for RIA-805. The recovery was higher than 95% in both assays. No cross-reaction was observed with members of aspartic proteinase family as well as with other tested proteins. In both RIA-780 and RIA-805, inhibition of the binding of the tracer by antisera was parallel between standard curve and serial dilutions of pregnant ewe samples. In conclusion, the two homologous RIA systems are suitable for early quantification of ovPAG concentrations in ewe plasma samples from day 18 after breeding.

Original languageEnglish
Pages (from-to)257-262
Number of pages6
JournalReproduction in Domestic Animals
Volume42
Issue number3
DOIs
Publication statusPublished - Jun 2007

Fingerprint

glycoproteins
Sheep
Glycoproteins
pregnancy
sheep
Pregnancy
tracer techniques
ewes
nimesulide
sampling
Breeding
antiserum
Immune Sera
assays
Aspartic Acid Proteases
aspartic proteinases
Artificial Insemination
Cross Reactions
breeding
antibody formation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)
  • Reproductive Medicine

Cite this

El Amiri, B., Karen, A., Sulon, J., Melo De Sousa, N., Alvarez-Oxiley, A. V., Cognié, Y., ... Beckers, J. F. (2007). Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in lacaune sheep. Reproduction in Domestic Animals, 42(3), 257-262. https://doi.org/10.1111/j.1439-0531.2006.00761.x

Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in lacaune sheep. / El Amiri, B.; Karen, A.; Sulon, J.; Melo De Sousa, N.; Alvarez-Oxiley, A. V.; Cognié, Y.; Szenci, O.; Beckers, J. F.

In: Reproduction in Domestic Animals, Vol. 42, No. 3, 06.2007, p. 257-262.

Research output: Contribution to journalArticle

El Amiri, B, Karen, A, Sulon, J, Melo De Sousa, N, Alvarez-Oxiley, AV, Cognié, Y, Szenci, O & Beckers, JF 2007, 'Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in lacaune sheep', Reproduction in Domestic Animals, vol. 42, no. 3, pp. 257-262. https://doi.org/10.1111/j.1439-0531.2006.00761.x
El Amiri, B. ; Karen, A. ; Sulon, J. ; Melo De Sousa, N. ; Alvarez-Oxiley, A. V. ; Cognié, Y. ; Szenci, O. ; Beckers, J. F. / Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in lacaune sheep. In: Reproduction in Domestic Animals. 2007 ; Vol. 42, No. 3. pp. 257-262.
@article{4ab4ca9f31d4481dabd68bec02ff335a,
title = "Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in lacaune sheep",
abstract = "This study describes ovine pregnancy-associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi-purified ovPAG as standard, tracer and immunogens for antibody production in rabbits. Antisera R780 (against ovPAG57+59kDa) and R805 (against ovPAG558+61kDa) were used respectively in RIA-780 and RIA-805. Blood samples were collected at days 0, 18, 20, 22 and 25 after artificial insemination. From day 18 after breeding onward, the mean ovPAG concentration was significantly higher (p <0.001) in plasma samples from pregnant ewes (n = 17) than in non-pregnant ones (n = 3). The specific activity of the tracer was 11 760 Ci/mmol in RIA-780 and 14 900 Ci/mmol in RIA-805. The minimal detection limits for RIA-780 and RIA-805 were 0.2 ng/ml and 0.3 ng/ml, respectively. The intra-assay CV of samples with low (1.0 ng/ml), medium (2.5 ng/ml) and high (4.0 ng/ml) PAG concentrations were 3{\%}, 6{\%} and 9{\%} for RIA-780 and 8{\%}, 9{\%} and 5{\%} for RIA-805. The inter-assay CV in the same samples were 13{\%}, 12{\%} and 7{\%} for RIA-780 and 13{\%}, 11{\%} and 5{\%} for RIA-805. The recovery was higher than 95{\%} in both assays. No cross-reaction was observed with members of aspartic proteinase family as well as with other tested proteins. In both RIA-780 and RIA-805, inhibition of the binding of the tracer by antisera was parallel between standard curve and serial dilutions of pregnant ewe samples. In conclusion, the two homologous RIA systems are suitable for early quantification of ovPAG concentrations in ewe plasma samples from day 18 after breeding.",
author = "{El Amiri}, B. and A. Karen and J. Sulon and {Melo De Sousa}, N. and Alvarez-Oxiley, {A. V.} and Y. Cogni{\'e} and O. Szenci and Beckers, {J. F.}",
year = "2007",
month = "6",
doi = "10.1111/j.1439-0531.2006.00761.x",
language = "English",
volume = "42",
pages = "257--262",
journal = "Reproduction in Domestic Animals",
issn = "0936-6768",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in lacaune sheep

AU - El Amiri, B.

AU - Karen, A.

AU - Sulon, J.

AU - Melo De Sousa, N.

AU - Alvarez-Oxiley, A. V.

AU - Cognié, Y.

AU - Szenci, O.

AU - Beckers, J. F.

PY - 2007/6

Y1 - 2007/6

N2 - This study describes ovine pregnancy-associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi-purified ovPAG as standard, tracer and immunogens for antibody production in rabbits. Antisera R780 (against ovPAG57+59kDa) and R805 (against ovPAG558+61kDa) were used respectively in RIA-780 and RIA-805. Blood samples were collected at days 0, 18, 20, 22 and 25 after artificial insemination. From day 18 after breeding onward, the mean ovPAG concentration was significantly higher (p <0.001) in plasma samples from pregnant ewes (n = 17) than in non-pregnant ones (n = 3). The specific activity of the tracer was 11 760 Ci/mmol in RIA-780 and 14 900 Ci/mmol in RIA-805. The minimal detection limits for RIA-780 and RIA-805 were 0.2 ng/ml and 0.3 ng/ml, respectively. The intra-assay CV of samples with low (1.0 ng/ml), medium (2.5 ng/ml) and high (4.0 ng/ml) PAG concentrations were 3%, 6% and 9% for RIA-780 and 8%, 9% and 5% for RIA-805. The inter-assay CV in the same samples were 13%, 12% and 7% for RIA-780 and 13%, 11% and 5% for RIA-805. The recovery was higher than 95% in both assays. No cross-reaction was observed with members of aspartic proteinase family as well as with other tested proteins. In both RIA-780 and RIA-805, inhibition of the binding of the tracer by antisera was parallel between standard curve and serial dilutions of pregnant ewe samples. In conclusion, the two homologous RIA systems are suitable for early quantification of ovPAG concentrations in ewe plasma samples from day 18 after breeding.

AB - This study describes ovine pregnancy-associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi-purified ovPAG as standard, tracer and immunogens for antibody production in rabbits. Antisera R780 (against ovPAG57+59kDa) and R805 (against ovPAG558+61kDa) were used respectively in RIA-780 and RIA-805. Blood samples were collected at days 0, 18, 20, 22 and 25 after artificial insemination. From day 18 after breeding onward, the mean ovPAG concentration was significantly higher (p <0.001) in plasma samples from pregnant ewes (n = 17) than in non-pregnant ones (n = 3). The specific activity of the tracer was 11 760 Ci/mmol in RIA-780 and 14 900 Ci/mmol in RIA-805. The minimal detection limits for RIA-780 and RIA-805 were 0.2 ng/ml and 0.3 ng/ml, respectively. The intra-assay CV of samples with low (1.0 ng/ml), medium (2.5 ng/ml) and high (4.0 ng/ml) PAG concentrations were 3%, 6% and 9% for RIA-780 and 8%, 9% and 5% for RIA-805. The inter-assay CV in the same samples were 13%, 12% and 7% for RIA-780 and 13%, 11% and 5% for RIA-805. The recovery was higher than 95% in both assays. No cross-reaction was observed with members of aspartic proteinase family as well as with other tested proteins. In both RIA-780 and RIA-805, inhibition of the binding of the tracer by antisera was parallel between standard curve and serial dilutions of pregnant ewe samples. In conclusion, the two homologous RIA systems are suitable for early quantification of ovPAG concentrations in ewe plasma samples from day 18 after breeding.

UR - http://www.scopus.com/inward/record.url?scp=34248589318&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248589318&partnerID=8YFLogxK

U2 - 10.1111/j.1439-0531.2006.00761.x

DO - 10.1111/j.1439-0531.2006.00761.x

M3 - Article

VL - 42

SP - 257

EP - 262

JO - Reproduction in Domestic Animals

JF - Reproduction in Domestic Animals

SN - 0936-6768

IS - 3

ER -