Measurement of formaldehyde and some fully N-methylated substances in tissue cultures of Datura innoxia

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18 Citations (Scopus)

Abstract

Formaldehyde (HCHO) and choline, trigonelline and N(ε)-trimethyl-L- lysine (TML) were detected and measured in tissue cultures of Datura innoxia Mill. by TLC and OPLC. The higher level of HCHO in young tissues appears to originate from the methylation reactions. This is in correlation with the high level of fully N-methylated substances. In spite of this the level of the fully N-methylated substances decreased with the aging of tissue. HCHO level also decreased with aging. However, in old tissues the amount of HCHO again increased. This HCHO originates mainly from demethylation reactions. It seems that there is a dynamic relationship between methylation/demethylation processes based on HCHO reactions and cell proliferation.

Original languageEnglish
Pages (from-to)317-320
Number of pages4
JournalPlant Growth Regulation
Volume20
Issue number3
Publication statusPublished - 1996

Fingerprint

Datura
formaldehyde
Formaldehyde
tissue culture
methylation
trigonelline
Methylation
choline
cell proliferation
lysine
Choline
Lysine
Cell Proliferation
tissues

Keywords

  • aging
  • cell proliferation
  • choline
  • Datura innoxia Mill.
  • formaldehyde
  • methylation-demethylation
  • N(ε)-trimethyl-L- lysine
  • OPLC
  • tissue culture
  • TLC
  • trigonelline

ASJC Scopus subject areas

  • Plant Science

Cite this

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title = "Measurement of formaldehyde and some fully N-methylated substances in tissue cultures of Datura innoxia",
abstract = "Formaldehyde (HCHO) and choline, trigonelline and N(ε)-trimethyl-L- lysine (TML) were detected and measured in tissue cultures of Datura innoxia Mill. by TLC and OPLC. The higher level of HCHO in young tissues appears to originate from the methylation reactions. This is in correlation with the high level of fully N-methylated substances. In spite of this the level of the fully N-methylated substances decreased with the aging of tissue. HCHO level also decreased with aging. However, in old tissues the amount of HCHO again increased. This HCHO originates mainly from demethylation reactions. It seems that there is a dynamic relationship between methylation/demethylation processes based on HCHO reactions and cell proliferation.",
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T1 - Measurement of formaldehyde and some fully N-methylated substances in tissue cultures of Datura innoxia

AU - Tyihák, E.

AU - Szőke, E.

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AB - Formaldehyde (HCHO) and choline, trigonelline and N(ε)-trimethyl-L- lysine (TML) were detected and measured in tissue cultures of Datura innoxia Mill. by TLC and OPLC. The higher level of HCHO in young tissues appears to originate from the methylation reactions. This is in correlation with the high level of fully N-methylated substances. In spite of this the level of the fully N-methylated substances decreased with the aging of tissue. HCHO level also decreased with aging. However, in old tissues the amount of HCHO again increased. This HCHO originates mainly from demethylation reactions. It seems that there is a dynamic relationship between methylation/demethylation processes based on HCHO reactions and cell proliferation.

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