Measurement of erythrocyte deformability and methodological adaptation for small-animal microsurgical models

N. Németh, Adrienn Gulyas, Anita Balint, Katalin Peto, E. Bráth, Ferenc Kiss, I. Furka, Oguz K. Baskurt, I. Mikó

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Measuring erythrocyte deformability with bulk filtrometers is common, but there are problems with standardization and sample quantity in small laboratory animals. In this study, blood was drawn from mice, rats, and dogs, and then erythrocyte suspensions at 1-5% hematocrit were prepared. Bulk filtrometers with a 5- or 3-μm pore-diameter filter were used to determine the initial relative filtration rate (IRFR) and relative cell transit time (RCTT). Hematological composition was also tested. In all species, the highest IRFR values occurred at 1% hematocrit, and the lowest values at 5%. In mice and rats, RCTT values were lowest at 1% and highest at 5% using a 5-μm filter, whereas in dogs, RCTT was elevated at 1-2%. Using a 3-μm filter, each species showed the same phenomena under 3%. RCTT values may become distorted above a certain cell-size/pore-size ratio. In mice and rats, 1% suspension is applicable for bulk filtrometry, but only using 5-μm filters.

Original languageEnglish
Pages (from-to)33-37
Number of pages5
JournalMicrosurgery
Volume26
Issue number1
DOIs
Publication statusPublished - 2006

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Erythrocyte Deformability
Animal Models
Hematocrit
Suspensions
Dogs
Laboratory Animals
Cell Size
Erythrocytes

ASJC Scopus subject areas

  • Surgery

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Measurement of erythrocyte deformability and methodological adaptation for small-animal microsurgical models. / Németh, N.; Gulyas, Adrienn; Balint, Anita; Peto, Katalin; Bráth, E.; Kiss, Ferenc; Furka, I.; Baskurt, Oguz K.; Mikó, I.

In: Microsurgery, Vol. 26, No. 1, 2006, p. 33-37.

Research output: Contribution to journalArticle

Németh, N. ; Gulyas, Adrienn ; Balint, Anita ; Peto, Katalin ; Bráth, E. ; Kiss, Ferenc ; Furka, I. ; Baskurt, Oguz K. ; Mikó, I. / Measurement of erythrocyte deformability and methodological adaptation for small-animal microsurgical models. In: Microsurgery. 2006 ; Vol. 26, No. 1. pp. 33-37.
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