Measurement of contrast transfer function in super-resolution microscopy using two-color fluorescence dip spectroscopy

Yoshinori Iketaki, Takeshi Watanabe, N. Bokor, Takashige Omatsu, Takashi Hiraga, Kimihisa Yamamoto, Masaaki Fujii

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The contrast transfer function (CTF) of super-resolution microscopy was quantitatively investigated using a fluorescent scale. The scale has minute fluorescent line patterns, finer than 100 nm, and is suitable for measuring CTF in super-resolution microscopy. The measured CTF shows that super-resolution microscopy can indeed improve the optical properties of fluorescent images and enable us to observe a structure with the spatial resolution overcoming the diffraction limit. From the CTF, it has been found that super-resolution microcopy can resolve a 100 nm line-and-space pattern and provides a contrast of 10%. The CTF corresponds to a PSF with a full-width at half-maximum (FWHM) of 130 nm. An evaluation using a 100 nmΦ fluorescent bead consistently supports the results given by the CTF for super-resolution microscopy.

Original languageEnglish
Pages (from-to)6-10
Number of pages5
JournalApplied Spectroscopy
Volume61
Issue number1
Publication statusPublished - Jan 2007

Fingerprint

Fluorescence spectroscopy
transfer functions
Transfer functions
Microscopic examination
microscopy
Color
color
fluorescence
spectroscopy
Full width at half maximum
beads
Optical properties
Diffraction
spatial resolution
optical properties
evaluation
diffraction

Keywords

  • Contrast transfer function
  • Fluorescence- depletion process
  • Fluorescent scale
  • Rhodamine 6G
  • Super-resolution microscopy
  • Two-color dip spectroscopy

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation

Cite this

Iketaki, Y., Watanabe, T., Bokor, N., Omatsu, T., Hiraga, T., Yamamoto, K., & Fujii, M. (2007). Measurement of contrast transfer function in super-resolution microscopy using two-color fluorescence dip spectroscopy. Applied Spectroscopy, 61(1), 6-10.

Measurement of contrast transfer function in super-resolution microscopy using two-color fluorescence dip spectroscopy. / Iketaki, Yoshinori; Watanabe, Takeshi; Bokor, N.; Omatsu, Takashige; Hiraga, Takashi; Yamamoto, Kimihisa; Fujii, Masaaki.

In: Applied Spectroscopy, Vol. 61, No. 1, 01.2007, p. 6-10.

Research output: Contribution to journalArticle

Iketaki, Y, Watanabe, T, Bokor, N, Omatsu, T, Hiraga, T, Yamamoto, K & Fujii, M 2007, 'Measurement of contrast transfer function in super-resolution microscopy using two-color fluorescence dip spectroscopy', Applied Spectroscopy, vol. 61, no. 1, pp. 6-10.
Iketaki, Yoshinori ; Watanabe, Takeshi ; Bokor, N. ; Omatsu, Takashige ; Hiraga, Takashi ; Yamamoto, Kimihisa ; Fujii, Masaaki. / Measurement of contrast transfer function in super-resolution microscopy using two-color fluorescence dip spectroscopy. In: Applied Spectroscopy. 2007 ; Vol. 61, No. 1. pp. 6-10.
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