Measurement of antibody binding to antigenic peptides conjugated in situ to albumin-coated microtitre plates

M. R. Price, M. Sekowski, D. S W Hooi, L. G. Durrant, F. Hudecz, S. J B Tendler

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Monoclonal antibodies have been prepared against a synthetic peptide with a sequence corresponding to a repeated hydrophilic region of the protein core of the human MUC-2 gastrointestinal mucin. Peptide conjugates, prepared by glutaraldehyde cross-linking with keyhole limpet haemocyanin (KLH) and bovine serum albumin (BSA), were employed as the immunogen and target antigen (for screening by ELISA), respectively. However, for the measurement of antibody binding to peptide by an ELISA procedure, an alternative strategy was developed and is described in this report: peptides were conjugated directly to BSA immobilized by physical adsorption to the surface of microtitre plate wells. This procedure permits peptides to be tested as target antigens by ELISA without prior preparation of peptide-carrier conjugates.

Original languageEnglish
Pages (from-to)277-281
Number of pages5
JournalJournal of Immunological Methods
Volume159
Issue number1-2
DOIs
Publication statusPublished - Feb 26 1993

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Albumins
Peptides
Antibodies
Enzyme-Linked Immunosorbent Assay
Bovine Serum Albumin
Mucin-2
Antigens
Glutaral
Adsorption
Monoclonal Antibodies

Keywords

  • Antigenic peptide
  • Conjugation to bovine serum albumin
  • ELISA

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Measurement of antibody binding to antigenic peptides conjugated in situ to albumin-coated microtitre plates. / Price, M. R.; Sekowski, M.; Hooi, D. S W; Durrant, L. G.; Hudecz, F.; Tendler, S. J B.

In: Journal of Immunological Methods, Vol. 159, No. 1-2, 26.02.1993, p. 277-281.

Research output: Contribution to journalArticle

Price, M. R. ; Sekowski, M. ; Hooi, D. S W ; Durrant, L. G. ; Hudecz, F. ; Tendler, S. J B. / Measurement of antibody binding to antigenic peptides conjugated in situ to albumin-coated microtitre plates. In: Journal of Immunological Methods. 1993 ; Vol. 159, No. 1-2. pp. 277-281.
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