MeaB-dependent nutrition sensing regulates autolysis in carbon starved Aspergillus nidulans cultures

Melinda Szilágyi, István Pócsi, Katalin Forgács, Tamás Emri

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Carbon starvation induced autolysis is an active process of self-digestion and is under complex regulation in Aspergillus nidulans. In this study we investigated how autolysis depends on the composition of the culture medium, especially on the presence of yeast extract. We demonstrated that the rate of autolytic cell wall degradation as well as the extracellular chitinase and proteinase productions significantly decreased in the presence of this nutrient. The effect of yeast extract on carbon starved cultures was independent of loss-of-function mutations in the carbon and nitrogen regulatory genes creA and areA and in the heterotrimeric G protein signalling genes fadA and ganB. In contrast, the nitrogen regulating transcription factor MeaB was involved in the yeast-extract-mediated repression of autolysis. Reverse transcriptase - polymerase chain reaction (RT-PCR) experiments demonstrated that MeaB affects the FluG-BrlA sporulation regulatory pathway by affecting transcription of brlA, a gene also initiating the autolytic cell wall degradation in this fungus.

Original languageEnglish
Pages (from-to)104-108
Number of pages5
JournalIndian Journal of Microbiology
Volume50
Issue number1
DOIs
Publication statusPublished - 2010

Keywords

  • Aspergillus nidulans
  • Autolysis
  • Carbon starvation
  • Chitinase
  • MeaB

ASJC Scopus subject areas

  • Microbiology

Fingerprint Dive into the research topics of 'MeaB-dependent nutrition sensing regulates autolysis in carbon starved Aspergillus nidulans cultures'. Together they form a unique fingerprint.

  • Cite this