Matrix/analyte ratio influencing polymer molecular weight distribution in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

G. Schlosser, Annamária Jakab, Gabriella Pocsfalvi, K. Vékey, F. Hudecz, G. Mező

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been used to characterize poly(L-lysine) polymers and unique oligomer peptides, like 10-, 15- and 25- mer [Lys] n oligolysine peptides. Several matrices have been tried in order to find optimal conditions, but only α-cyano-4-hydroxycinnamic acid gave analytically useful spectra. The synthetic oligomers and their mixtures gave good quality spectra, showing protonated and cationized molecules, including doubly charged species. The polymers, analogously, gave a wide distribution of single- and double-cationized peak series. The polymer distributions observed indicate the presence of significant suppression effects. The concentration (matrix/analyte ratio) was found to influence the results significantly; distributions shifting to higher masses when higher polymer concentrations were used. This effect was studied in detail using the synthetic ('monodisperse') oligolysine peptides. It was found that the relative intensities change by over an order of magnitude in the 0.1-10 pmol/μL concentration range (typical for most proteomic analyses). The results indicate that concentration effects need to be considered when MALDI-MS is used for quantitative purposes.

Original languageEnglish
Pages (from-to)1249-1254
Number of pages6
JournalRapid Communications in Mass Spectrometry
Volume23
Issue number9
DOIs
Publication statusPublished - May 15 2009

Fingerprint

Molecular weight distribution
Ionization
Mass spectrometry
Desorption
Polymers
Lasers
Oligomers
Peptides
Lysine
Molecules

ASJC Scopus subject areas

  • Spectroscopy
  • Analytical Chemistry
  • Organic Chemistry

Cite this

@article{54e8e593837e4c5bb9ca211a955a10fd,
title = "Matrix/analyte ratio influencing polymer molecular weight distribution in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry",
abstract = "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been used to characterize poly(L-lysine) polymers and unique oligomer peptides, like 10-, 15- and 25- mer [Lys] n oligolysine peptides. Several matrices have been tried in order to find optimal conditions, but only α-cyano-4-hydroxycinnamic acid gave analytically useful spectra. The synthetic oligomers and their mixtures gave good quality spectra, showing protonated and cationized molecules, including doubly charged species. The polymers, analogously, gave a wide distribution of single- and double-cationized peak series. The polymer distributions observed indicate the presence of significant suppression effects. The concentration (matrix/analyte ratio) was found to influence the results significantly; distributions shifting to higher masses when higher polymer concentrations were used. This effect was studied in detail using the synthetic ('monodisperse') oligolysine peptides. It was found that the relative intensities change by over an order of magnitude in the 0.1-10 pmol/μL concentration range (typical for most proteomic analyses). The results indicate that concentration effects need to be considered when MALDI-MS is used for quantitative purposes.",
author = "G. Schlosser and Annam{\'a}ria Jakab and Gabriella Pocsfalvi and K. V{\'e}key and F. Hudecz and G. Mező",
year = "2009",
month = "5",
day = "15",
doi = "10.1002/rcm.3993",
language = "English",
volume = "23",
pages = "1249--1254",
journal = "Rapid Communications in Mass Spectrometry",
issn = "0951-4198",
publisher = "John Wiley and Sons Ltd",
number = "9",

}

TY - JOUR

T1 - Matrix/analyte ratio influencing polymer molecular weight distribution in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

AU - Schlosser, G.

AU - Jakab, Annamária

AU - Pocsfalvi, Gabriella

AU - Vékey, K.

AU - Hudecz, F.

AU - Mező, G.

PY - 2009/5/15

Y1 - 2009/5/15

N2 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been used to characterize poly(L-lysine) polymers and unique oligomer peptides, like 10-, 15- and 25- mer [Lys] n oligolysine peptides. Several matrices have been tried in order to find optimal conditions, but only α-cyano-4-hydroxycinnamic acid gave analytically useful spectra. The synthetic oligomers and their mixtures gave good quality spectra, showing protonated and cationized molecules, including doubly charged species. The polymers, analogously, gave a wide distribution of single- and double-cationized peak series. The polymer distributions observed indicate the presence of significant suppression effects. The concentration (matrix/analyte ratio) was found to influence the results significantly; distributions shifting to higher masses when higher polymer concentrations were used. This effect was studied in detail using the synthetic ('monodisperse') oligolysine peptides. It was found that the relative intensities change by over an order of magnitude in the 0.1-10 pmol/μL concentration range (typical for most proteomic analyses). The results indicate that concentration effects need to be considered when MALDI-MS is used for quantitative purposes.

AB - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been used to characterize poly(L-lysine) polymers and unique oligomer peptides, like 10-, 15- and 25- mer [Lys] n oligolysine peptides. Several matrices have been tried in order to find optimal conditions, but only α-cyano-4-hydroxycinnamic acid gave analytically useful spectra. The synthetic oligomers and their mixtures gave good quality spectra, showing protonated and cationized molecules, including doubly charged species. The polymers, analogously, gave a wide distribution of single- and double-cationized peak series. The polymer distributions observed indicate the presence of significant suppression effects. The concentration (matrix/analyte ratio) was found to influence the results significantly; distributions shifting to higher masses when higher polymer concentrations were used. This effect was studied in detail using the synthetic ('monodisperse') oligolysine peptides. It was found that the relative intensities change by over an order of magnitude in the 0.1-10 pmol/μL concentration range (typical for most proteomic analyses). The results indicate that concentration effects need to be considered when MALDI-MS is used for quantitative purposes.

UR - http://www.scopus.com/inward/record.url?scp=66449107572&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66449107572&partnerID=8YFLogxK

U2 - 10.1002/rcm.3993

DO - 10.1002/rcm.3993

M3 - Article

C2 - 19306286

AN - SCOPUS:66449107572

VL - 23

SP - 1249

EP - 1254

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

IS - 9

ER -