Matrix metalloproteinase-9 expression in the normal mucosa-adenoma- dysplasia-adenocarcinoma sequence of the colon

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Abstract

It has been proposed that matrix metalloproteinases (MMPs) play a role in tumor invasion. We determined protein expression of matrix metalloproteinase-9 (MMP-9) in colorectal cancer (CRC), corresponding normal mucosa and colorectal adenomas. For confirmation of immunohistochemical results MMP-9 TaqMan RT-PCR analysis was performed. Expression of MMP-9 was determined on paraffin embedded biopsy sections by immunohistochemistry in 31 CRC patients (from cancer tissue and corresponding normal mucosa) and in 30 patients with adenoma (nine adenomas with high grade of dysplasia). MMP-9 immunostaining was determined semi-quantitatively. For Taqman RT-PCR analyses normal mucosa (n=5), adenoma without (n=6) and with high grade dysplasia (n=7) and CRC (n=10) were investigated. Statistical analysis with ANOVA, LSD test and correlation analysis were performed. P value of

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalPathology and Oncology Research
Volume14
Issue number1
DOIs
Publication statusPublished - Mar 2008

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Matrix Metalloproteinase 9
Adenoma
Colon
Mucous Membrane
Adenocarcinoma
Colorectal Neoplasms
Lysergic Acid Diethylamide
Polymerase Chain Reaction
Matrix Metalloproteinases
Paraffin
Neoplasms
Analysis of Variance
Immunohistochemistry
Biopsy
Proteins

Keywords

  • Adenoma
  • Colorectal cancer
  • Dysplasia
  • Matrix metalloproteinase-9
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

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abstract = "It has been proposed that matrix metalloproteinases (MMPs) play a role in tumor invasion. We determined protein expression of matrix metalloproteinase-9 (MMP-9) in colorectal cancer (CRC), corresponding normal mucosa and colorectal adenomas. For confirmation of immunohistochemical results MMP-9 TaqMan RT-PCR analysis was performed. Expression of MMP-9 was determined on paraffin embedded biopsy sections by immunohistochemistry in 31 CRC patients (from cancer tissue and corresponding normal mucosa) and in 30 patients with adenoma (nine adenomas with high grade of dysplasia). MMP-9 immunostaining was determined semi-quantitatively. For Taqman RT-PCR analyses normal mucosa (n=5), adenoma without (n=6) and with high grade dysplasia (n=7) and CRC (n=10) were investigated. Statistical analysis with ANOVA, LSD test and correlation analysis were performed. P value of",
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author = "L. Hersz{\'e}nyi and F. S{\'i}pos and O. Galamb and N. Solymosi and I. Hritz and P. Miheller and L. Berczi and B. Moln{\'a}r and Z. Tulassay",
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AU - Herszényi, L.

AU - Sípos, F.

AU - Galamb, O.

AU - Solymosi, N.

AU - Hritz, I.

AU - Miheller, P.

AU - Berczi, L.

AU - Molnár, B.

AU - Tulassay, Z.

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AB - It has been proposed that matrix metalloproteinases (MMPs) play a role in tumor invasion. We determined protein expression of matrix metalloproteinase-9 (MMP-9) in colorectal cancer (CRC), corresponding normal mucosa and colorectal adenomas. For confirmation of immunohistochemical results MMP-9 TaqMan RT-PCR analysis was performed. Expression of MMP-9 was determined on paraffin embedded biopsy sections by immunohistochemistry in 31 CRC patients (from cancer tissue and corresponding normal mucosa) and in 30 patients with adenoma (nine adenomas with high grade of dysplasia). MMP-9 immunostaining was determined semi-quantitatively. For Taqman RT-PCR analyses normal mucosa (n=5), adenoma without (n=6) and with high grade dysplasia (n=7) and CRC (n=10) were investigated. Statistical analysis with ANOVA, LSD test and correlation analysis were performed. P value of

KW - Adenoma

KW - Colorectal cancer

KW - Dysplasia

KW - Matrix metalloproteinase-9

KW - Polymerase chain reaction

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