Mathematical modelling of single-line flow-injection analysis systems with single-layer enzyme electrode detection Part 3. Experimental verification of the model

Spas D. Kolev, G. Nagy, Ernö Pungor

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Glucose and urea electrodes, prepared by two different enzyme immobilization techniques and used as detectors in a single-line flow-injection manifold, were experimentally investigated for elucidating the influence of their most important parameters, i.e., the initial substrate concentration in the sample, the enzyme concentration in the reaction layer and its thickness and the buffer concentration, on the output signal. The results obtained were compared with the theoretical predictions based on simulations of the model for single-line flow-injection systems with single-layer enzyme electrode detection. The good qualitative agreement which was observed is a convincing experimental verification of this model and of the guidelines for the production of flow-through biocatalytic electrodes with optimum design based upon it.

Original languageEnglish
Pages (from-to)167-175
Number of pages9
JournalAnalytica Chimica Acta
Volume254
Issue number1-2
DOIs
Publication statusPublished - Nov 20 1991

Fingerprint

Enzyme electrodes
Flow Injection Analysis
Biosensing Techniques
Urea electrodes
systems analysis
Electrodes
electrode
Theoretical Models
enzyme
Enzyme immobilization
Injections
Enzymes
Immobilization
modeling
Urea
Buffers
Guidelines
Detectors
Glucose
immobilization

Keywords

  • Enzyme electrodes
  • Flow system
  • Glucose
  • Mathematical modelling
  • Urea

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Mathematical modelling of single-line flow-injection analysis systems with single-layer enzyme electrode detection Part 3. Experimental verification of the model. / Kolev, Spas D.; Nagy, G.; Pungor, Ernö.

In: Analytica Chimica Acta, Vol. 254, No. 1-2, 20.11.1991, p. 167-175.

Research output: Contribution to journalArticle

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