Mass-transfer properties of insulin on core-shell and fully porous stationary phases

Nándor Lambert, Ibolya Kiss, Attila Felinger

Research output: Contribution to journalArticle

11 Citations (Scopus)


The mass-transfer properties of three superficially-porous packing materials, with 2.6 and 3.6. μm particle and 1.9, 2.6, and 3.2. μm inner core diameter, respectively, were investigated and compared with those of fully porous packings with similar particle properties. Several sources of band spreading in the chromatographic bed have been identified and studied according to the general rate model of chromatography. Besides the axial dispersion in the stream of the mobile phase, and the external mass transfer resistance, the intraparticle diffusion was studied in depth. The first absolute and the second central moments of the peaks of human insulin, over a wide range of mobile phase velocities were measured and used for the calculation of the mass-transfer coefficients. The experimental data were also analyzed using the stochastic or molecular dynamic model of Giddings and Eyring. The dissimilarities of the mass-transfer observed in the different columns were identified and evaluated.

Original languageEnglish
Pages (from-to)84-91
Number of pages8
JournalJournal of Chromatography A
Publication statusPublished - Oct 31 2014


  • Core-shell particles
  • General rate model
  • Insulin
  • Mass transfer
  • Stochastic theory

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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