Mass spectrometry of extracellular vesicles

Gabriella Pocsfalvi, Christopher Stanly, Annalisa Vilasi, Immacolata Fiume, Giovambattista Capasso, Lilla Turiák, E. Búzás, K. Vékey

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The review briefly summaries main features of extracellular vesicles, a joint terminology for exosomes, microvesicles, and apoptotic vesicles. These vesicles are in the center of interest in biology and medical sciences, and form a very active field of research. Mass spectrometry (MS), with its specificity and sensitivity, has the potential to identify and characterize molecular composition of these vesicles; but as yet there are only a limited, but fast-growing, number of publications that use MS workflows in this field. MS is the major tool to assess protein composition of extracellular vesicles: qualitative and quantitative proteomics approaches are both reviewed. Beside proteins, lipid and metabolite composition of vesicles might also be best assessed by MS techniques; however there are few applications as yet in this respect. The role of alternative analytical approaches, like gel-based proteomics and antibody-based immunoassays, are also mentioned. The objective of the review is to give an overview of this fast-growing field to help orient MS-based research on extracellular vesicles.

Original languageEnglish
Pages (from-to)3-21
Number of pages19
JournalMass Spectrometry Reviews
Volume35
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

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Mass spectrometry
Mass Spectrometry
mass spectroscopy
Proteomics
Chemical analysis
medical science
Exosomes
proteins
immunoassay
terminology
Workflow
metabolites
Terminology
Metabolites
antibodies
biology
Immunoassay
Research
lipids
Publications

Keywords

  • biomarker discovery
  • extracellular vesicles
  • lipids
  • proteins
  • subcellular proteomics

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Analytical Chemistry
  • Spectroscopy
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Pocsfalvi, G., Stanly, C., Vilasi, A., Fiume, I., Capasso, G., Turiák, L., ... Vékey, K. (2016). Mass spectrometry of extracellular vesicles. Mass Spectrometry Reviews, 35(1), 3-21. https://doi.org/10.1002/mas.21457

Mass spectrometry of extracellular vesicles. / Pocsfalvi, Gabriella; Stanly, Christopher; Vilasi, Annalisa; Fiume, Immacolata; Capasso, Giovambattista; Turiák, Lilla; Búzás, E.; Vékey, K.

In: Mass Spectrometry Reviews, Vol. 35, No. 1, 01.01.2016, p. 3-21.

Research output: Contribution to journalArticle

Pocsfalvi, G, Stanly, C, Vilasi, A, Fiume, I, Capasso, G, Turiák, L, Búzás, E & Vékey, K 2016, 'Mass spectrometry of extracellular vesicles', Mass Spectrometry Reviews, vol. 35, no. 1, pp. 3-21. https://doi.org/10.1002/mas.21457
Pocsfalvi G, Stanly C, Vilasi A, Fiume I, Capasso G, Turiák L et al. Mass spectrometry of extracellular vesicles. Mass Spectrometry Reviews. 2016 Jan 1;35(1):3-21. https://doi.org/10.1002/mas.21457
Pocsfalvi, Gabriella ; Stanly, Christopher ; Vilasi, Annalisa ; Fiume, Immacolata ; Capasso, Giovambattista ; Turiák, Lilla ; Búzás, E. ; Vékey, K. / Mass spectrometry of extracellular vesicles. In: Mass Spectrometry Reviews. 2016 ; Vol. 35, No. 1. pp. 3-21.
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