Mass spectrometry-based salivary proteomics for the discovery of head and neck squamous cell carcinoma

Tamas Jarai, Gabor Maasz, Andras Burian, Agnes Bona, Eva Jambor, Imre Gerlinger, L. Márk

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The 5-year survival rates for cases of head and neck squamous cell carcinoma (HNSCC) are only some 60%, mainly because 20%-40% of the patients develop a local relapse in the same or an adjacent anatomic region, even when the surgical margins are histologically tumour-free. Tumours are often discovered in an advanced stage because of the lack of specific symptoms and the diagnostic difficulties. The more advanced the stage of the tumour, the more invasive the diagnostic and treatment interventions needed. An early molecular diagnosis is therefore of vital importance in order to increase the survival rate. The aimof this study was to develop an efficient rapid and sensitive mass spectrometric method for the detection of differentially expressed proteins as tumourspecific biomarkers in saliva from HNSCC patients. Whole saliva samples were collected from patients with HNSCC and from healthy subjects. The proteins were profiled by using SDS PAGE, MALDI TOF/TOF mass spectrometry and the Mascot database search engine. Several potential tumour markers were identified, including annexin A1, beta- and gamma-actin, cytokeratin 4 and 13, zinc finger proteins and P53 pathway proteins. All of these proteins play a proven role in tumour genesis, and have not been detected previously in saliva. Salivary proteomics is a non-invasive specific method for cancer diagnosis and follow-up treatment. It provides facilities for the readily reproducible and reliable detection of tumours in early stages.

Original languageEnglish
Pages (from-to)623-628
Number of pages6
JournalPathology and Oncology Research
Volume18
Issue number3
DOIs
Publication statusPublished - Jul 2012

Fingerprint

Proteomics
Mass Spectrometry
Saliva
Neoplasms
Proteins
Actins
Keratin-4
Keratin-13
Survival Rate
Annexin A1
Search Engine
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Zinc Fingers
Tumor Biomarkers
Carcinoma, squamous cell of head and neck
Polyacrylamide Gel Electrophoresis
Early Diagnosis
Healthy Volunteers
Biomarkers
Databases

Keywords

  • Biomarker discovery
  • MALDI TOF MS
  • Saliva
  • Tumor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Mass spectrometry-based salivary proteomics for the discovery of head and neck squamous cell carcinoma. / Jarai, Tamas; Maasz, Gabor; Burian, Andras; Bona, Agnes; Jambor, Eva; Gerlinger, Imre; Márk, L.

In: Pathology and Oncology Research, Vol. 18, No. 3, 07.2012, p. 623-628.

Research output: Contribution to journalArticle

Jarai, Tamas ; Maasz, Gabor ; Burian, Andras ; Bona, Agnes ; Jambor, Eva ; Gerlinger, Imre ; Márk, L. / Mass spectrometry-based salivary proteomics for the discovery of head and neck squamous cell carcinoma. In: Pathology and Oncology Research. 2012 ; Vol. 18, No. 3. pp. 623-628.
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