Mass spectrometric proteome analysis for profiling temperature-dependent changes of protein expression in wild-type Caenorhabditis elegans

A. Mádi, Stefan Mikkat, Bruno Ringel, Markus Ulbrich, Hans Jürgen Thiesen, Michael O. Glocker

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We investigated the effect of cultivation temperatures on the protein expression levels in the fourth larval stage of the postembryonic development of wild-type Caenorhabditis elegans by mass spectrometric proteome analysis. From the 64 protein spots that were investigated, 5 spots were found reproducibly differently expressed when proteome maps derived from animals kept at 15°C and at 25°C, respectively, were compared. Spots of heat shock proteins HSP 70 (CE18679 or CE09682) and HSP 16 (CE14249) were present only in gels from protein extracts when worms were grown at 15°C. Spots of two metabolic enzymes, the isocitrate dehydrogenase (CE10345) and the aspartic proteinase (CE21681) were detected only in cultures grown at the lower temperature as well. A protein with still unknown function (CE05036) was present only in gels from worm samples grown at 25°C. We show for the first time by proteome analyses that cultivation of worms at the lowest temperature of the known physiological range (15°C) already triggers a (weak) stress response in wild-type animals. This work led to the identification of "internal control proteins" in the wild-type strain for further characterization of temperature-sensitive strains using a proteomics approach.

Original languageEnglish
Pages (from-to)1526-1534
Number of pages9
JournalProteomics
Volume3
Issue number8
DOIs
Publication statusPublished - Aug 1 2003

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Caenorhabditis elegans
Proteome
Temperature
Proteins
Animals
Gels
Aspartic Acid Proteases
Isocitrate Dehydrogenase
HSP70 Heat-Shock Proteins
Wild Animals
Heat-Shock Proteins
Proteomics
Enzymes

Keywords

  • Caenorhabditis elegans
  • Fourth larval stage
  • Ionization-time of flight-mass spectrometry
  • Matrix assisted laser desorption
  • Temperature-sensitive strains

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Mass spectrometric proteome analysis for profiling temperature-dependent changes of protein expression in wild-type Caenorhabditis elegans. / Mádi, A.; Mikkat, Stefan; Ringel, Bruno; Ulbrich, Markus; Thiesen, Hans Jürgen; Glocker, Michael O.

In: Proteomics, Vol. 3, No. 8, 01.08.2003, p. 1526-1534.

Research output: Contribution to journalArticle

Mádi, A. ; Mikkat, Stefan ; Ringel, Bruno ; Ulbrich, Markus ; Thiesen, Hans Jürgen ; Glocker, Michael O. / Mass spectrometric proteome analysis for profiling temperature-dependent changes of protein expression in wild-type Caenorhabditis elegans. In: Proteomics. 2003 ; Vol. 3, No. 8. pp. 1526-1534.
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