Mass spectrometric investigation of alamethicin

Tamás Marik, Csaba Várszegi, László Kredics, Csaba Vágvölgyi, András Szekeres

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The peptaibols, such as alamethicin are secondary metabolites belonging to the family of fungal peptide antibiotics. These compounds are linear, amphipathic oligopeptides classified into 4 subfamilies and composed of 5-20 amino acids. Their backbones usually contain several nonproteinogenic amino acid residues representing characteristic building blocks of the structure. In our present work, the mass spectrometric analysis of alamethicin has been investigated by on-line reversed-phase high performance liquid chromatography (HPLC) coupled to electrospray ionization tandem mass spectrometry (ES-MS). Initially, the parameters of the MS were optimized by the continuous infusion of an alamethicin standard solution directly into the ESI source. Then, the proper HPLC method was developed for the analysis of the alamethicin components, which was capable to separate the peaks of F50-5, F50-6a, F50-7 and F50-8b from each other, which could be identified based on their mass spectra.

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalActa Biologica Szegediensis
Volume57
Issue number2
Publication statusPublished - 2013

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Keywords

  • Alamethicin
  • Mass spectrometry
  • Peptaibol
  • RP-HPLC-UV/ESI-IT-MS
  • Αaminoisobutyric acid

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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