Mass spectrometric identification of ancient proteins as potential molecular biomarkers for a 2000-year-old osteogenic sarcoma

Agnes Bona, Zoltan Papai, Gabor Maasz, Gabor A. Toth, Eva Jambor, Janos Schmidt, Csaba Toth, Csilla Farkas, L. Márk

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Osteosarcoma is the most common primary malignant tumor of bone usually occurring in young adolescent and children. This disease has a poor prognosis, because of the metastases in the period of tumor progression, which are usually developed previous to the clinical diagnosis. In this paper, a 2000-year-old ancient bone remain with osteogenic sarcoma was analyzed searching for tumor biomarkers which are closely related to this disease. After a specific extraction SDS-PAGE gel electrophoresis followed by tryptic digestion was performed. After the digestion the samples were measured using MALDI TOF/TOF MS. Healthy bone samples from same archaeological site were used as control samples. Our results show that in the pathological skeletal remain several well known tumor biomarkers are detected such as annexin A10, BCL-2-like protein, calgizzarin, rho GTPase-activating protein 7, HSP beta-6 protein, transferrin and vimentin compared to the control samples. The identified protein biomarkers can be useful in the discovery of malignant bone lesions such as osteosarcoma in the very early stage of the disease from paleoanthropological remains.

Original languageEnglish
Article numbere87215
JournalPLoS One
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 27 2014

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sarcoma
Biomarkers
Osteosarcoma
biomarkers
Bone
bones
Bone and Bones
neoplasms
osteosarcoma
Tumor Biomarkers
Tumors
Digestion
Proteins
proteins
digestion
GTPase-activating proteins
Annexins
sampling
vimentin
antineoplaston A10

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mass spectrometric identification of ancient proteins as potential molecular biomarkers for a 2000-year-old osteogenic sarcoma. / Bona, Agnes; Papai, Zoltan; Maasz, Gabor; Toth, Gabor A.; Jambor, Eva; Schmidt, Janos; Toth, Csaba; Farkas, Csilla; Márk, L.

In: PLoS One, Vol. 9, No. 1, e87215, 27.01.2014.

Research output: Contribution to journalArticle

Bona, Agnes ; Papai, Zoltan ; Maasz, Gabor ; Toth, Gabor A. ; Jambor, Eva ; Schmidt, Janos ; Toth, Csaba ; Farkas, Csilla ; Márk, L. / Mass spectrometric identification of ancient proteins as potential molecular biomarkers for a 2000-year-old osteogenic sarcoma. In: PLoS One. 2014 ; Vol. 9, No. 1.
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