Mass spectrometric and linear discriminant analysis of N-glycans of human serum alpha-1-acid glycoprotein in cancer patients and healthy individuals

Tímea Imre, Tibor Kremmer, Károly Héberger, Éva Molnár-Szöllosi, Krisztina Ludányi, Gabriella Pócsfalvi, Antonio Malorni, László Drahos, Károly Vékey

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

N-glycan oligosaccharides of human serum α1-acid glycoprotein (AGP) samples isolated from 43 individuals (healthy individuals and patients with lymphoma and with ovarian tumor) were analyzed by MALDI-TOF mass spectrometry and a multivariate statistical method (linear discriminant analysis, LDA). 34 different glycan structures have been identified. From the glycosylation pattern determined by mass spectrometry fucosylation and branching indices have been calculated. These parameters show only small differences between the patient groups studied, but these differences are not sufficiently large to use as a potential biomarker. LDA analysis, on the other hand shows a very good separation between the three groups (with a classification of 88%). Cross-validation indicates that the method has predictive power: Identifying cancerous vs. healthy individuals shows 96% selectivity and 93% specificity; identification of lymphoma vs. the mixed group of healthy and ovarian tumor cases is also promising (72% selectivity and 84% specificity). The pilot study presented here demonstrates that mass spectrometry combined with linear discriminant analysis (LDA) may provide valuable data for identifying and studying the pathophysiology of malignant diseases.

Original languageEnglish
Pages (from-to)186-197
Number of pages12
JournalJournal of proteomics
Volume71
Issue number2
DOIs
Publication statusPublished - Jul 21 2008

Keywords

  • Alpha-1-acid glycoprotein (AGP)
  • Biomarker
  • Cancer
  • Glycosylation
  • Linear discriminant analysis (LDA)
  • Lymphoma
  • MALDI-TOF
  • Mass spectrometry
  • Ovarian tumor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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