Mass isolation of plant chromosomes and nuclei

Gy Hadlaczky, Gy Bisztray, Tünde Praznovszky, D. Dudits

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A method is presented for mass isolation of metaphase chromosomes and nuclei from plant protoplasts. The isolation procedure was developed for both monocotyledonous and dicotyledonous species using wheat (Triticum monococcum) and poppy (Papaver somniferum) cell cultures. Metaphase chromosomes were isolated from partially synchronized mitotic protoplasts, while for the isolation of nuclei unsynchronized protoplasts were used. Light and electron-microscopic studies revealed that isolated chromosomes and nuclei preserved their intact morphology. A preliminary biochemical study of chromosomal proteins was made by polyacrylamide-gel electrophoresis. Because of the purity and high quantity of isolated chromosomes and nuclei, the given isolation procedure can supply useful material for structural and biochemical studies, and for genetic manipulation.

Original languageEnglish
Pages (from-to)278-285
Number of pages8
JournalPlanta
Volume157
Issue number3
DOIs
Publication statusPublished - Apr 1 1983

Keywords

  • Chromosomal proteins
  • Chromosome isolation
  • Nucleus isolation
  • Papaver
  • Protein, chromosomal
  • Triticum (nuclei isolation)

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Fingerprint Dive into the research topics of 'Mass isolation of plant chromosomes and nuclei'. Together they form a unique fingerprint.

  • Cite this

    Hadlaczky, G., Bisztray, G., Praznovszky, T., & Dudits, D. (1983). Mass isolation of plant chromosomes and nuclei. Planta, 157(3), 278-285. https://doi.org/10.1007/BF00405195