Marker profile, enzyme activity, and function of a human myelomonocytic leukemia cell line

Mária Kávai, R. Ádány, Gabriella Pásti, Péter Surányi, G. Szűcs, L. Muszbek, Ferenc Boján, G. Szegedi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Morphological and functional characteristics of a permanent human leukemia cell line (DD) that possesses myelomonocytic features were investigated. The cells bear a second type Fcγ receptor and form rosettes with sheep erythrocytes sensitized with rabbit IgG (EA). However, the surface-bound EA is not internalized. The cell line lacks the surface markers CD2, CD 19, CD 14, HLADR, Fcγ receptor I, Fc/gg receptor III, and CR3. α1-Antitrypsin, lysozyme, Factor XIII a subunit of blood coagulation, and acid phosphatase reactions were negative. A terminal differentiation of the DD cell line was observed when the expression of CD 14, CR3, Fcγ receptor I, and Fcγ receptor III was induced. The DD cells induced with 12-O-tetradecanoylphorbol-13-acetate or Escherichia coli lipopolysaccharide can internalize EA via Fcγ receptor II and complement-coated yeast in the function of the inducers. The phagocytic ability appears to be parallel with the appearance of enzymes which participate in phagocytosis.

Original languageEnglish
Pages (from-to)531-540
Number of pages10
JournalCellular Immunology
Volume139
Issue number2
DOIs
Publication statusPublished - 1992

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Fc Receptors
Leukemia
Cell Line
Enzymes
Macrophage-1 Antigen
Factor XIII
Blood Coagulation
Tetradecanoylphorbol Acetate
Muramidase
Acid Phosphatase
Phagocytosis
Lipopolysaccharides
Sheep
Immunoglobulin G
Yeasts
Erythrocytes
Escherichia coli
Rabbits

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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Marker profile, enzyme activity, and function of a human myelomonocytic leukemia cell line. / Kávai, Mária; Ádány, R.; Pásti, Gabriella; Surányi, Péter; Szűcs, G.; Muszbek, L.; Boján, Ferenc; Szegedi, G.

In: Cellular Immunology, Vol. 139, No. 2, 1992, p. 531-540.

Research output: Contribution to journalArticle

Kávai, Mária ; Ádány, R. ; Pásti, Gabriella ; Surányi, Péter ; Szűcs, G. ; Muszbek, L. ; Boján, Ferenc ; Szegedi, G. / Marker profile, enzyme activity, and function of a human myelomonocytic leukemia cell line. In: Cellular Immunology. 1992 ; Vol. 139, No. 2. pp. 531-540.
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