Marker profile, enzyme activity, and function of a human myelomonocytic leukemia cell line

Mária Kávai, Róza Ádány, Gabriella Pásti, Péter Surányi, Gabriella Szücs, László Muszbek, Ferenc Boján, Gyula Szegedi

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12 Citations (Scopus)

Abstract

Morphological and functional characteristics of a permanent human leukemia cell line (DD) that possesses myelomonocytic features were investigated. The cells bear a second type Fcγ receptor and form rosettes with sheep erythrocytes sensitized with rabbit IgG (EA). However, the surface-bound EA is not internalized. The cell line lacks the surface markers CD2, CD 19, CD 14, HLADR, Fcγ receptor I, Fc/gg receptor III, and CR3. α1-Antitrypsin, lysozyme, Factor XIII a subunit of blood coagulation, and acid phosphatase reactions were negative. A terminal differentiation of the DD cell line was observed when the expression of CD 14, CR3, Fcγ receptor I, and Fcγ receptor III was induced. The DD cells induced with 12-O-tetradecanoylphorbol-13-acetate or Escherichia coli lipopolysaccharide can internalize EA via Fcγ receptor II and complement-coated yeast in the function of the inducers. The phagocytic ability appears to be parallel with the appearance of enzymes which participate in phagocytosis.

Original languageEnglish
Pages (from-to)531-540
Number of pages10
JournalCellular Immunology
Volume139
Issue number2
DOIs
Publication statusPublished - Feb 1992

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ASJC Scopus subject areas

  • Immunology

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