Marker construction and cloning of a cutl-like sequence with ARS activity in the fission yeast Schizosaccharomyces japonicus

Aniko Bozsik, Z. Szilagyi, Z. Benko, M. Sipiczki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The dimorphic fission yeast Schizosaccharomyces japonicus has proved to be an excellent experimental model for the investigation of the eukaryotic cell. Here we show that it has a haplontic life cycle, in which the diploid phase is confined to the zygote. To make it amenable to genetic and molecular analysis, we generated genetic markers and cloned a genomic sequence which acts as ars when integrated into a plasmid. Diploids suitable for testing complementation and recombination between markers can be formed by protoplast fusion. The complementation tests and the recombination frequencies determined in octads of spores identified 28 non-allelic groups (genes) of mutations of the auxotrophic and mycelium-negative mutants. Two groups of linked markers were also identified. The cloned fragment, which expresses ars activity, encodes a putative amino acid sequence highly similar to a conserved domain of proteins Cut1 (Schizosaccharomyces pombe), BimB (Aspergillus nidulans) and Esp1 (Saccharomyces cerevisiae).

Original languageEnglish
Pages (from-to)485-498
Number of pages14
JournalYeast
Volume19
Issue number6
DOIs
Publication statusPublished - 2002

Fingerprint

Schizosaccharomyces
Schizosaccharomyces pombe
Cloning
Diploidy
Yeast
Genetic Recombination
Organism Cloning
molecular cloning
diploidy
Schizosaccharomyces pombe Proteins
Genetic Complementation Test
Aspergillus nidulans
protoplast fusion
Protoplasts
Zygote
Aspergillus
Mycelium
Eukaryotic Cells
zygote
Life Cycle Stages

Keywords

  • Ars
  • Auxotrophy
  • Cut1
  • Dimorphism
  • Fission yeast
  • Octad
  • Protoplast
  • Transformation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Marker construction and cloning of a cutl-like sequence with ARS activity in the fission yeast Schizosaccharomyces japonicus. / Bozsik, Aniko; Szilagyi, Z.; Benko, Z.; Sipiczki, M.

In: Yeast, Vol. 19, No. 6, 2002, p. 485-498.

Research output: Contribution to journalArticle

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