Mapping the genomic binding sites of the activated retinoid X receptor in murine bone marrow-derived macrophages using chromatin immunoprecipitation sequencing

Bence Daniel, Balint L. Balint, Zsuzsanna S. Nagy, L. Nagy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chromatin immunoprecipitation followed by massively parallel sequencing (ChIP-Seq) is a powerful technique to map the genomic location of a given chromatin bound factor (i.e., transcription factors, cofactors) or epigenetic marks, such as histone modifi cation. The procedure is based on cross-linking of proteins to DNA followed by the capture of the protein-DNA complexes by “ChIP-grade” antibodies. In this chapter we describe in detail the experimental method developed in our laboratory to investigate in vivo the DNA- binding characteristics of a key heterodimeric nuclear receptor, the retinoid X receptor (RXR) in murine bone marrow-derived macrophages.

Original languageEnglish
Pages (from-to)15-24
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume1204
DOIs
Publication statusPublished - 2014

Fingerprint

Retinoid X Receptors
Chromatin Immunoprecipitation
Macrophages
Binding Sites
DNA
High-Throughput Nucleotide Sequencing
Cytoplasmic and Nuclear Receptors
Epigenomics
Histones
Chromatin
Cations
Proteins
Transcription Factors
Antibodies

Keywords

  • Binding site
  • ChIP
  • Chromatin
  • Cistrome
  • Macrophage
  • RXR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

@article{26fdc6039eeb4216ad5bdeb9264a3f8e,
title = "Mapping the genomic binding sites of the activated retinoid X receptor in murine bone marrow-derived macrophages using chromatin immunoprecipitation sequencing",
abstract = "Chromatin immunoprecipitation followed by massively parallel sequencing (ChIP-Seq) is a powerful technique to map the genomic location of a given chromatin bound factor (i.e., transcription factors, cofactors) or epigenetic marks, such as histone modifi cation. The procedure is based on cross-linking of proteins to DNA followed by the capture of the protein-DNA complexes by “ChIP-grade” antibodies. In this chapter we describe in detail the experimental method developed in our laboratory to investigate in vivo the DNA- binding characteristics of a key heterodimeric nuclear receptor, the retinoid X receptor (RXR) in murine bone marrow-derived macrophages.",
keywords = "Binding site, ChIP, Chromatin, Cistrome, Macrophage, RXR",
author = "Bence Daniel and Balint, {Balint L.} and Nagy, {Zsuzsanna S.} and L. Nagy",
year = "2014",
doi = "10.1007/978-1-4939-1346-6_2",
language = "English",
volume = "1204",
pages = "15--24",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Mapping the genomic binding sites of the activated retinoid X receptor in murine bone marrow-derived macrophages using chromatin immunoprecipitation sequencing

AU - Daniel, Bence

AU - Balint, Balint L.

AU - Nagy, Zsuzsanna S.

AU - Nagy, L.

PY - 2014

Y1 - 2014

N2 - Chromatin immunoprecipitation followed by massively parallel sequencing (ChIP-Seq) is a powerful technique to map the genomic location of a given chromatin bound factor (i.e., transcription factors, cofactors) or epigenetic marks, such as histone modifi cation. The procedure is based on cross-linking of proteins to DNA followed by the capture of the protein-DNA complexes by “ChIP-grade” antibodies. In this chapter we describe in detail the experimental method developed in our laboratory to investigate in vivo the DNA- binding characteristics of a key heterodimeric nuclear receptor, the retinoid X receptor (RXR) in murine bone marrow-derived macrophages.

AB - Chromatin immunoprecipitation followed by massively parallel sequencing (ChIP-Seq) is a powerful technique to map the genomic location of a given chromatin bound factor (i.e., transcription factors, cofactors) or epigenetic marks, such as histone modifi cation. The procedure is based on cross-linking of proteins to DNA followed by the capture of the protein-DNA complexes by “ChIP-grade” antibodies. In this chapter we describe in detail the experimental method developed in our laboratory to investigate in vivo the DNA- binding characteristics of a key heterodimeric nuclear receptor, the retinoid X receptor (RXR) in murine bone marrow-derived macrophages.

KW - Binding site

KW - ChIP

KW - Chromatin

KW - Cistrome

KW - Macrophage

KW - RXR

UR - http://www.scopus.com/inward/record.url?scp=84921736532&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84921736532&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-1346-6_2

DO - 10.1007/978-1-4939-1346-6_2

M3 - Article

C2 - 25182757

AN - SCOPUS:84921736532

VL - 1204

SP - 15

EP - 24

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -