Mapping Helper Virus Functions for Cucumber Mosaic Virus Satellite RNA with Pseudorecombinants Derived from Cucumber Mosaic and Tomato Aspermy Viruses

Enrique Moriones, Isabel Diaz, Beatriz Fernández-Cuartero, Aurora Fraile, Jósef Burgyn, Fernando García-Arenal

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Abstract

P-TAV is a strain of tomato aspermy virus (TAV) able to efficiently support the systemic accumulation of some (i.e., B2-satRNA) but not of other (i, e., Ix-satRNA) strains of the satellite RNA (satRNA) of cucumber mosaic virus (CMV) in both tobacco and in tomato. As reported for V-TAV, the failure to support the systemic accumulation of Ix-satRNA seems to be due to an inefficient support of its systemic movement. Pseudorecombinants obtained by the exchange of RNAs 1 + 2 between P-TAV and TrK7-CMV, an efficient helper for the systemic accumulation of Ix-satRNA, were assayed for their ability to support the accumulation of CMV-satRNAs in tobacco plants and protoplasts. Pseudorecombinants having RNAs 1 + 2 from CMV supported the systemic movement and accumulation of CMV-satRNA as efficiently as CMV, whereas pseudorecombinants having RNAs 1 + 2 from TAV supported the CMV-satRNA very poorly. Thus, the ability to support the systemic movement and accumulation of CMV-satRNA is determined primarily by RNAs 1 + 2 and not by RNA 3, which is presumed to encode movement functions in the cucumoviruses and only has a minor, modulating effect on the systemic accumulation of satRNA. This suggests that for systemic movement CMV-satRNA has to interact with (the gene products of) RNAs 1 and/or 2 or that these viral RNAs compete with the satRNA for interaction with the coat or other movement proteins.

Original languageEnglish
Article number71682
Pages (from-to)574-577
Number of pages4
JournalVirology
Volume205
Issue number2
DOIs
Publication statusPublished - Dec 1994

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ASJC Scopus subject areas

  • Virology

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