Mammalian retinal horizontal cells are unconventional GABAergic neurons

Sercan Deniz, Eric Wersinger, Yannick Schwab, Carole Mura, Ferenc Erdelyi, Gábor Szabó, Alvaro Rendon, José Alain Sahel, Serge Picaud, Michel J. Roux

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Lateral interactions at the first retinal synapse have been initially proposed to involve GABA by transporter-mediated release from horizontal cells, onto GABAA receptors expressed on cone photoreceptor terminals and/or bipolar cell dendrites. However, in the mammalian retina, horizontal cells do not seem to contain GABA systematically or to express membrane GABA transporters. We here report that mouse retinal horizontal cells express GAD65 and/or GAD67 mRNA, and were weakly but consistently immunostained for GAD65/67. While GABA was readily detected after intracardiac perfusion, it was lost during classical preparation for histology or electrophysiology. It could not be restored by incubation in a GABA-containing medium, confirming the absence of membrane GABA transporters in these cells. However, GABA was synthesized de novo from glutamate or glutamine, upon addition of pyridoxal 5′-phosphate, a cofactor of GAD65/67. Mouse horizontal cells are thus atypical GABAergic neurons, with no functional GABA uptake, but a glutamate and/or glutamine transport system allowing GABA synthesis, probably depending physiologically from glutamate released by photoreceptors. Our results suggest that the role of GABA in lateral inhibition may have been underestimated, at least in mammals, and that tissue pre-incubation with glutamine and pyridoxal 5′-phosphate should yield a more precise estimate of outer retinal processing.

Original languageEnglish
Pages (from-to)350-362
Number of pages13
JournalJournal of neurochemistry
Volume116
Issue number3
DOIs
Publication statusPublished - Feb 1 2011

Keywords

  • horizontal cell
  • inhibitory transmission
  • mouse
  • neurotransmitter transporter
  • retina
  • vesicular release

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Mammalian retinal horizontal cells are unconventional GABAergic neurons'. Together they form a unique fingerprint.

  • Cite this

    Deniz, S., Wersinger, E., Schwab, Y., Mura, C., Erdelyi, F., Szabó, G., Rendon, A., Sahel, J. A., Picaud, S., & Roux, M. J. (2011). Mammalian retinal horizontal cells are unconventional GABAergic neurons. Journal of neurochemistry, 116(3), 350-362. https://doi.org/10.1111/j.1471-4159.2010.07114.x