Mammalian polymerase ζ is essential for post-replication repair of UV-induced DNA lesions

Jacob G. Jansen, Anastasia Tsaalbi-Shtylik, Giel Hendriks, Johan Verspuy, Himabindu Gali, L. Haracska, Niels de Wind

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

DNA polymerase ζ is believed to be an essential constituent of DNA damage tolerance, comprising several pathways that allow the replication of DNA templates containing unrepaired damage. We wanted to better define the role of polymerase ζ in DNA damage tolerance in mammalian cells. To this aim we have investigated replication of ultraviolet light-damaged DNA templates in mouse embryonic fibroblasts deficient for Rev3, the catalytic subunit of polymerase ζ. We found that Rev3 is important for a post-replication repair pathway of helix-distorting [6-4]pyrimidine-pyrimidone photoproducts and, to a lesser extent, of cyclobutane pyrimidine dimers. Unlike its partner Rev1, Rev3 appears not to be involved in an immediate translesion synthesis pathway at a stalled replication fork. The deficiency of Rev3-/- MEFs in post-replication repair of different photoproducts contributes to the extreme sensitivity of these cells to UV light.

Original languageEnglish
Pages (from-to)1444-1451
Number of pages8
JournalDNA Repair
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 3 2009

Fingerprint

Ultraviolet Rays
DNA Damage
Repair
Pyrimidine Dimers
Damage tolerance
DNA
DNA-Directed DNA Polymerase
DNA Replication
Catalytic Domain
Fibroblasts
Ultraviolet radiation
Cells
pyrimidine-pyrimidone dimer

Keywords

  • DNA damage bypass
  • DNA translesion synthesis
  • Mammalian cells
  • Post-replication repair
  • Rev3
  • Ultraviolet light

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Jansen, J. G., Tsaalbi-Shtylik, A., Hendriks, G., Verspuy, J., Gali, H., Haracska, L., & de Wind, N. (2009). Mammalian polymerase ζ is essential for post-replication repair of UV-induced DNA lesions. DNA Repair, 8(12), 1444-1451. https://doi.org/10.1016/j.dnarep.2009.09.006

Mammalian polymerase ζ is essential for post-replication repair of UV-induced DNA lesions. / Jansen, Jacob G.; Tsaalbi-Shtylik, Anastasia; Hendriks, Giel; Verspuy, Johan; Gali, Himabindu; Haracska, L.; de Wind, Niels.

In: DNA Repair, Vol. 8, No. 12, 03.12.2009, p. 1444-1451.

Research output: Contribution to journalArticle

Jansen, JG, Tsaalbi-Shtylik, A, Hendriks, G, Verspuy, J, Gali, H, Haracska, L & de Wind, N 2009, 'Mammalian polymerase ζ is essential for post-replication repair of UV-induced DNA lesions', DNA Repair, vol. 8, no. 12, pp. 1444-1451. https://doi.org/10.1016/j.dnarep.2009.09.006
Jansen, Jacob G. ; Tsaalbi-Shtylik, Anastasia ; Hendriks, Giel ; Verspuy, Johan ; Gali, Himabindu ; Haracska, L. ; de Wind, Niels. / Mammalian polymerase ζ is essential for post-replication repair of UV-induced DNA lesions. In: DNA Repair. 2009 ; Vol. 8, No. 12. pp. 1444-1451.
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