Mammalian phosphatidylinositol 4-kinases

Ludwig M G Heilmeyer, György Vereb, G. Vereb, Annamária Kakuk, Ilona Szivák

Research output: Contribution to journalArticle

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Abstract

Three phosphatidylinositol 4-kinase isoforms, PI4K 230, 92 and 55 have been cloned and sequenced allowing a much wider characterization than the previously employed enzymological typing into type II and III enzymes. PI4K 230 and 92 contain a highly conserved catalytic core, PI4K55 one with a much lower degree of similarity. Candidate kinase motifs, deduced from the protein kinase super family, are absolutely conserved in all isoforms. Kinase activities are described based on their sensitivity and reactivity towards wortmannin, phenylarsine oxide (PAO) and 5′-p-fluorosulfonylbenzoyladenosine (FSBA). Localization of all isoforms in the cell is reported. All enzymes contain nuclear localization and export sequence motifs (NLS and NES) leading to the expectation that they can be transferred to the nucleus. PI4K230 has been found in the nucleolus, PI4K92 in the nucleus, additionally further broadening the function of these enzymes. In the cytoplasm of neuronal cells, PI4K230 is distributed evenly on membranes that are ultra structurally cisterns of the rough endoplasmatic reticulum, outer membranes of mitochondria, multivesicular bodies, and are in close vicinity of synaptic contacts. PI4K92 is functionally characterized as a key enzyme regulating Golgi disintegration/reorganization during mitosis probably via phosphorylation by cyclin-dependent kinases on well-defined sites. PI4K55 is involved in the production of second messengers, diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (InsP3) at the plasma membrane, moreover, in the endocytotic pathway in the cytoplasm.

Original languageEnglish
Pages (from-to)59-65
Number of pages7
JournalIUBMB Life
Volume55
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

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1-Phosphatidylinositol 4-Kinase
Protein Isoforms
Enzymes
Cytoplasm
Phosphotransferases
Multivesicular Bodies
Membranes
Reticulum
Phosphorylation
Mitochondria
Inositol 1,4,5-Trisphosphate
Cell Nucleus Active Transport
Cyclin-Dependent Kinases
Disintegration
Diglycerides
Second Messenger Systems
Cell membranes
Mitosis
Protein Kinases
Catalytic Domain

Keywords

  • Brefeldin A
  • Leptomycin B
  • Nuclear localization and export signals
  • Nucleolar localization
  • Phosphatidylinositol 4-Kinase isoforms
  • Vesicular transport

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Heilmeyer, L. M. G., Vereb, G., Vereb, G., Kakuk, A., & Szivák, I. (2003). Mammalian phosphatidylinositol 4-kinases. IUBMB Life, 55(2), 59-65. https://doi.org/10.1080/1521654031000090896

Mammalian phosphatidylinositol 4-kinases. / Heilmeyer, Ludwig M G; Vereb, György; Vereb, G.; Kakuk, Annamária; Szivák, Ilona.

In: IUBMB Life, Vol. 55, No. 2, 01.02.2003, p. 59-65.

Research output: Contribution to journalArticle

Heilmeyer, LMG, Vereb, G, Vereb, G, Kakuk, A & Szivák, I 2003, 'Mammalian phosphatidylinositol 4-kinases', IUBMB Life, vol. 55, no. 2, pp. 59-65. https://doi.org/10.1080/1521654031000090896
Heilmeyer LMG, Vereb G, Vereb G, Kakuk A, Szivák I. Mammalian phosphatidylinositol 4-kinases. IUBMB Life. 2003 Feb 1;55(2):59-65. https://doi.org/10.1080/1521654031000090896
Heilmeyer, Ludwig M G ; Vereb, György ; Vereb, G. ; Kakuk, Annamária ; Szivák, Ilona. / Mammalian phosphatidylinositol 4-kinases. In: IUBMB Life. 2003 ; Vol. 55, No. 2. pp. 59-65.
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